Synonyms
Definition
Intestinal organoids are three-dimensional cultures of intestinal stem cells that reproduce the physiology and epithelial architecture of the intestine or colon.
Characteristics
Identification of Intestinal Stem Cell Markers
Adult stem cells are defined by (1) the ability to self-renew and persist lifelong (2) and the ability to differentiate into all cell types of a given tissue. Stem cell fate of a population can be identified by lineage tracing or by transplantation assays. In the case of the intestine, stem cells were long suspected to reside at the base of crypts, where proliferative cells reside. Based on the importance of the Wnt pathway in the proliferation and maintenance of the intestinal crypt, Wnt targets were screened as possible stem cell markers. The majority of Wnt genes are expressed in the crypt base as well as the transit-amplifying zone where progenitor cells lie; others are expressed in niche Paneth...
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References
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See Also
(2012) Organotypic Cultures. In: Schwab M (ed) Encyclopedia of Cancer, 3rd edn. Springer, Berlin/Heidelberg, p 2655. doi: 10.1007/978-3-642-16483-5_4254
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Roper, J., Yilmaz, Ö.H. (2014). Intestinal Organoids. In: Schwab, M. (eds) Encyclopedia of Cancer. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27841-9_7197-1
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DOI: https://doi.org/10.1007/978-3-642-27841-9_7197-1
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