Encyclopedia of Cancer

Living Edition
| Editors: Manfred Schwab

Live Cell Imaging

Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27841-9_7167-4

Definition

Today’s cancer research is increasingly focusing on dynamic processes like cell migration; morphological changes of cells, organs, or whole animals; and physiological events in living specimens in real time. An approach to address these issues is to employ several optical methods that are collectively called live-cell imaging. Live-cell imaging allows investigation of dynamic processes of living cells presented as a movie. Live-cell imaging provides spatial and temporal information of dynamic events in single cells, organotypic cultures, or whole organisms. These features make live-cell imaging a powerful technique for addressing important questions in cancer research.

Characteristics

Microscopic Techniques

The range of microscopic techniques applied for live-cell imaging is extremely wide. Often, the growth of cells or cell movement is observed over time using combined microscopes and contrasting methods such as phase contrast and differential interference contrast (DIC)....

Keywords

Fluorescence Resonance Energy Transfer Fluorescence Lifetime Fluorescence Recovery After Photobleaching Cyan Fluorescent Protein Fluorescent Lifetime Image Microscopy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Carloni V, Mazzocca A, Mello T, Galli A, Capaccioli S (2013) Cell fusion promotes chemoresistance in metastatic colon carcinoma. Oncogene 32(21):2649–2660. doi:10.1038/onc.2012.268Google Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Vinicio CarloniUniversity of FlorenceFlorenceItaly