Three-dimensional (3D) in vitro cultures closely resemble the in vivo environment with regard to cell shape and its inside and outside components, which can determine the behavior and gene expression of the cell. Reflecting natural conditions, these cultures allow for intercellular interactions with more realistic biochemical and physiological responses. In addition to the influence of this microenvironment on gene expression, cellular differentiation, proliferation, and apoptosis, 3D cultures allow the study of cell–cell and cell–extracellular matrix (ECM) interactions. Using human cells, these cultures avoid the costly and complex problems presented by animal models and in vivo studies, thereby circumventing unpredictable characteristics and requirements for ethical approval. These factors thus render human cells an obvious choice for many experimental applications.
Comparison of 3D and 2D Cultures
Since the advent...
KeywordsMammary Gland Mammary Epithelial Cell Tissue Culture Plastic Lumen Formation Acinar Structure
- Weaver VM, Fischer AH, Peterson OW, Bissell MJ (1996) The importance of the microenvironment in breast cancer progression: recapitulation of mammary tumorigenesis using a unique human mammary epithelial cell model and a three-dimensional culture assay. Biochem Cell Biol 74(6):833–851CrossRefPubMedPubMedCentralGoogle Scholar
- Weaver VM, Lelièvre S, Lakins JN, Chrenek MA, Jones JC, Giancotti F, Werb Z, Bissell MJ (2002) beta4 integrin-dependent formation of polarized three-dimensional architecture confers resistance to apoptosis in normal and malignant mammary epithelium. Cancer Cell 2(3):205–216CrossRefPubMedPubMedCentralGoogle Scholar