Encyclopedia of Cancer

Living Edition
| Editors: Manfred Schwab

Transgenic Mouse

  • Aparna Gupta
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27841-9_7098-5

Definition

A transgenic mouse contains additional, artificially introduced genetic material/DNA in every cell. The foreign DNA that has been purposely inserted into the mouse genome can be a foreign gene (not usually present in mice), a gene from the mouse, or a gene with a mutation (mutation: a change in the DNA code that alters the protein properties). The foreign gene is carefully constructed using recombinant DNA technology. In addition to the target gene itself, the DNA also includes other sequences that ensure:
  • Incorporation into the genomic DNA of the mouse

  • Proper expression of the gene by the cells

In order to incorporate the foreign DNA into every cell of the mouse, it has to be introduced into very early mouse embryo so that the germ cells (sperm and ovum) also receive the gene and pass it on to the next generation.

Characteristics

Methodology

There are two major methods that can be used to generate a transgenic mouse:
  • Pronuclear microinjection: Foreign DNA is introduced...

Keywords

Embryonic Stem Cell Inner Cell Mass loxP Site Conditional Gene Thymidine Kinase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

References

  1. Cameron ER (1997) Recent advances in transgenic technology. Mol Biotechnol 7(3):253–265CrossRefPubMedGoogle Scholar
  2. Cameron ER, Harvey MJ, Onions DE (1994) Transgenic science. Br Vet J 150(1):9–24CrossRefPubMedGoogle Scholar
  3. Kos CH (2004) Cre/loxP system for generating tissue-specific knockout mouse models. Nutr Rev 62(6 Pt 1):243–246PubMedGoogle Scholar
  4. Kuhn R, Torres RM (2002) Cre/loxP recombination system and gene targeting. Methods Mol Biol 180:175–204PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Life Science Research Associate, Department of Gastroenterology & HepatologyStanford University School of MedicineStanfordUSA