Encyclopedia of Cancer

Living Edition
| Editors: Manfred Schwab

Lentiviral Vectors

  • Lung-Ji Chang
  • Yingchi Chen
  • Chienling Chen
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27841-9_7088-3

Synonyms

Definition

Lentiviruses are members of the Retroviridae, which differ from the simple retroviruses in that they possess additional regulatory genes and elements that are not found in simple onco-retroviruses such as murine leukemia virus (MLV). The best known example of lentivirus is human immunodeficiency virus (HIV). Retroviral vectors have been popular because of their ability to integrate into host cell genome and maintain persistent gene expression. HIV-based lentiviral vector (LV) has been one of the most effective gene transfer vehicles for stable expression of cDNAs, shRNAs, antisense genes, etc., and vesicular stomatitis virus G-envelope (VSV-G) packaged LVs can target a broad spectrum of host cells including both dividing and nondividing cells.

Characteristics

Composition of LVs

An LV is composed of viral proteins and RNA genomes. The key viral proteins in an LV include Gag-Pol and Env. To generate an LV, a two-, three-, or four-plasmid system has...

Keywords

Chimeric Antigen Receptor Cell Vaccine Tumor Vaccine Major Splice Donor Negative Regulatory Signal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Chang LJ, Gay EE (2001) The molecular genetics of lentiviral vectors – current and future perspectives. Curr Gene Ther 1:237–251CrossRefPubMedGoogle Scholar
  2. Hu B, Tai A, Wang P (2011) Immunization delivered by lentiviral vectors for cancer and infectious diseases. Immunol Rev 239:45–61CrossRefPubMedPubMedCentralGoogle Scholar
  3. Porter DL, Levine BL, Kalow M, Bagg A, June CH (2011) Chimeric antigen receptor-modified T cells in chromic lymphoid leukemia. N Engl J Med 365:725–733CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2012

Authors and Affiliations

  1. 1.Department of Molecular Genetics and Microbiology, College of MedicineUniversity of FloridaGainesvilleUSA