Saporin is a ribosome-inactivating protein (RIP) from the common soapwort (Saponaria officinalis L.). It is an enzyme that releases a particular adenine from the 28S ribosomal RNA of eukaryotic ribosomes (at position 4324 referred to rat) and thus inactivates protein biosynthesis. Saporin is a type-I-RIP, which means that it consists only of one protein chain, the A-chain, representing catalytic activity, whereas type-II-RIPs contain in addition a cell-binding domain, the B-chain. Saporin is used in a number of attempts as part of targeted toxins in cancer therapy.
Structure and Function
Saporin is a ribosome-inactivating plant protein (RIP) from the common soapwort (Saponaria officinalis L.), a vespertine flower belonging to the carnation family (Caryophyllaceae) (Lombardi et al. 2010). The protein can be isolated from different tissues including leaves, stems, roots, flowers, and fruits. A number of isoforms differing in a couple of amino acids have been...
KeywordsMouse Tumor Model Endosomal Escape Eukaryotic Ribosome Tumor Necrosis Factor Receptor Family Dianthus Caryophyllus
- Gilabert-Oriol R, Weng A, Mallinckrodt Bv, Melzig MF, Fuchs H, Thakur M (2014) Immunotoxins constructed with ribosome-inactivating proteins and their enhancers: a lethal cocktail with tumor specific efficacy. Curr Pharm Des 20:6584–6643Google Scholar