Living reference work entry
Molecular tool ( Microarray (cDNA) Technology) developed during the early 1980s for molecular profiling of cancer cells for oncogene expression. In principle, this is a parallel analysis where any number of different oncogenes is spotted onto a matrix and fixed there. Then, total mRNA containing the oncogene mRNA transcripts is reverse transcribed into cDNA by using reverse transcriptase. During this process, the cDNA is labeled, either with a radioactive isotope or a fluorescent label, which is then hybridized to the OncoArray. The mRNA of a highly active oncogene is represented in the total mRNA at high abundancy. Consequently, more of these cDNA species will bind to the spotted oncogene and, upon further analysis, will produce higher signal intensity (Fig. 1). Early such molecular profiling had resulted in the identification of high expression, subsequent to amplification, of the gene MYCN (also N-MYC), which is related to the MYC oncogene, in a subgroup of human neuroblastoma...
KeywordsCancer Cell Signal Intensity Radioactive Isotope Reverse Transcriptase High Abundancy
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
- (2012) Molecular profiling. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 2363. doi: 10.1007/978-3-642-16483-5_7046Google Scholar
- (2012) MYCN. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, pp 2430–2431. doi: 10.1007/978-3-642-16483-5_3925Google Scholar
- (2012) Reverse transcriptase. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 3297. doi: 10.1007/978-3-642-16483-5_5086Google Scholar
© Springer-Verlag Berlin Heidelberg 2015