Encyclopedia of Cancer

Living Edition
| Editors: Manfred Schwab

Bioluminescence Imaging

  • Scott K. Lyons
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27841-9_640-3

Synonyms

BLI

Definition

A non-invasive preclinical imaging approach, reliant upon the detection of light from cells modified to express a luciferase transgene.

Characteristics

Bioluminescence imaging (BLI) is a preclinical imaging modality that has become popular with researchers across a broad range of biological disciplines. From an oncology perspective, the high sensitivity, versatility, speed and relative simplicity afforded by BLI has made it a particularly attractive imaging modality for measuring many aspects of in vivo tumor biology with cancer models.

In general terms and in comparison to the other preclincal imaging modalities, the hardware and consumable reagents needed for BLI are relatively cheap and safe (i.e., non-radioactive). In vivo images of multiple subjects can be acquired quickly (typically ranging between 1 and 180 s) so throughput is high. Moreover, BLI is arguable the most sensitive preclinical imaging approach available and in ideal experimental circumstances,...

Keywords

Renilla Luciferase Firefly Luciferase Luciferase Expression Bioluminescence Imaging Peptide Domain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Kocher B, Piwnica-Worms D (2013) Illuminating cancer systems with genetically engineered mouse models and coupled luciferase reporters in vivo. Cancer Discov 3(6):616–629PubMedPubMedCentralCrossRefGoogle Scholar
  2. Lyons SK, Patrick PS, Brindle KM (2013) Imaging mouse cancer models in vivo using reporter transgenes. Cold Spring Harbor Protoc 2013(8):685–699Google Scholar
  3. Patrick PS, Lyons SK, Rodrigues TB, Brindle KM (2014) Oatp1 enhances bioluminescence by acting as a plasma membrane transporter for D-luciferin. Mol Imaging Biol 16(5):626–634PubMedPubMedCentralCrossRefGoogle Scholar
  4. Mezzanotte L, Aswendt M, Tennstaedt A, Hoeben R, Hoehn M, Löwik C (2013) Evaluating reporter genes of different luciferases for optimized in vivo bioluminescence imaging of transplanted neural stem cells in the brain. Contrast Media Mol Imaging 8(6):505–513PubMedCrossRefGoogle Scholar

See Also

  1. (2012) Caspase. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, pp 674–675. doi:10.1007/978-3-642-16483-5_873Google Scholar
  2. (2012) Codon-optimization. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 891. doi:10.1007/978-3-642-16483-5_1249Google Scholar
  3. (2012) Cre/loxP. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 993. doi:10.1007/978-3-642-16483-5_1368Google Scholar
  4. (2012) CT. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, pp 1006–1007. doi:10.1007/978-3-642-16483-5_1398Google Scholar
  5. (2012) Enzyme. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 1259. doi:10.1007/978-3-642-16483-5_1917Google Scholar
  6. (2012) MRI. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 2382. doi:10.1007/978-3-642-16483-5_3854Google Scholar
  7. (2012) Spectral unmixing. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 3476. doi:10.1007/978-3-642-16483-5_5434Google Scholar
  8. (2012) S-phase. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 3476. doi:10.1007/978-3-642-16483-5_5138Google Scholar
  9. (2012) Steradian. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 3527. doi:10.1007/978-3-642-16483-5_5497Google Scholar
  10. (2012) Tumor Xenografts. In: Schwab M (ed) Encyclopedia of cancer, 3rd edn. Springer, Berlin/Heidelberg, p 3807. doi:10.1007/978-3-642-16483-5_6061Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Molecular Imaging GroupCRUK Cambridge Research Institute, Li Ka Shing CentreCambridgeUK