Encyclopedia of Cancer

Living Edition
| Editors: Manfred Schwab

Spectral Karyotyping

Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27841-9_5433-2

Definition

Spectral karyotyping (SKY) is a multi-fluorochrome fluorescence in situ hybridization technique (FISH) in which all the chromosome pairs are simultaneously visualized in different colors in a single hybridization. SKY determines the unique spectral profile of each chromosome generated by specific combinations of different fluorochromes. At the present time, SKY can be used to analyze human, mouse, and rat chromosomes.

Characteristics

Structural and numerical chromosomal alterations (aberrations) are the hallmarks of malignant diseases. Routine cytogenetic analysis based on G-banding techniques provides important information of diagnostic and prognostic relevance both in hematological malignancies and solid tumors. However, detection of chromosomal alterations by this method is complicated by the difficulty in routinely preparing metaphase spreads of sufficient quality and quantity, the clonal heterogeneity of the tumors, and the complexity of the many chromosomal...

Keywords

Painting Probe Spectral Karyotyping Probe Cocktail Apply Spectral Image Homogeneous Staining Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Bayani J, Squire JA (2001) Advances in the detection of chromosomal aberrations using spectral karyotyping. Clin Genet 59:65–75CrossRefPubMedGoogle Scholar
  2. Cohen N, Betts DR, Tavori U et al (2004) Karyotypic evolution pathways in medulloblastoma/primitive neuroectodermal tumor determined with a combination of spectral karyotyping, G-banding, and fluorescence in situ hybridization. Cancer Genet Cytogenet 149:44–52CrossRefPubMedGoogle Scholar
  3. Garini Y, Macville M, du Manoir S et al (1996) Spectral karyotyping. Bioimaging 4:65–72CrossRefGoogle Scholar
  4. Kakazu N, Abe T (2006) Multicolor banding technique, spectral color banding (SCAN): new development and applications. Cytogenet Genome Res 114:250–256CrossRefPubMedGoogle Scholar
  5. Oren Ben-Shoshan1 Sh, Simon A, Jacob-Hirsch J, Shaklai S, Paz-Yaacov1 N, Amariglio N, Rechavi G, Trakhtenbrot L (2014) Induction of polyploidy by nuclear fusion mechanism upon decreased expression of the nuclear envelope protein LAP2β in the human osteosarcoma cell line U2OS. Molecular Cytogenetics 7:9Google Scholar
  6. Saez B, Martin-Subero JI, Largo C et al (2006) Identification of recurrent chromosomal breakpoints in multiple myeloma with complex karyotypes by combined G-banding, spectral karyotyping and fluorescence in situ hybridization analysis. Cancer Genet Cytogenet 169:143–149CrossRefPubMedGoogle Scholar
  7. Shoshani O, Massalha H, Shani N, Kagan S, Ravid O, Madar S, Trakhtenbrot L, Leshkowitz D, Rechavi G, Zipori D (2012) Polyploidization of murine mesenchymal cells is associated with suppression of the long non-coding RNA H19 and reduced tumorigenicity. Cancer Res 15;72(24):6403–13Google Scholar
  8. Stanchescu R, Betts DR, Rechavi G, Amariglio N, Trakhtenbrot L (2009) Involvement of der(12)t(12;21)(p13;q22) and as well as additional rearrangements of chromosome 12 homolog in ETV6/RUNX1-positive acute lymphoblastic leukemia. Cancer Genet Cytogenet 1;190(1):26–32Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Molecular Cytogenetics LaboratoryInstitute of Hematology, The Chaim Sheba Medical CenterTel HashomerIsrael