Spectral karyotyping (SKY) is a multi-fluorochrome fluorescence in situ hybridization technique (FISH) in which all the chromosome pairs are simultaneously visualized in different colors in a single hybridization. SKY determines the unique spectral profile of each chromosome generated by specific combinations of different fluorochromes. At the present time, SKY can be used to analyze human, mouse, and rat chromosomes.
Structural and numerical chromosomal alterations (aberrations) are the hallmarks of malignant diseases. Routine cytogenetic analysis based on G-banding techniques provides important information of diagnostic and prognostic relevance both in hematological malignancies and solid tumors. However, detection of chromosomal alterations by this method is complicated by the difficulty in routinely preparing metaphase spreads of sufficient quality and quantity, the clonal heterogeneity of the tumors, and the complexity of the many chromosomal...
KeywordsPainting Probe Spectral Karyotyping Probe Cocktail Apply Spectral Image Homogeneous Staining Region
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