Encyclopedia of Cancer

Living Edition
| Editors: Manfred Schwab

Hollow Fiber Assay

  • Irene V. Bijnsdorp
  • Godefridus J. Peters
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27841-9_2786-2


The hollow fiber assay (HFA) is a fast in vivo assay to determine the cytotoxic effect of drugs, as well as their pharmacodynamic (Pharmacodynamics) effects on human tumor cell lines grown in hollow fibers that are implanted subcutaneously or intraperitoneally in mice or rats.


Various in vivo models exist to assess the efficacy of potential anticancer agents, including subcutaneously implanted xenografts, orthotopic models, and the HFA. The HFA has been optimized at the National Cancer Institute (NCI) and is a unique in vivo model for drug discovery. It permits the simultaneous evaluation of the efficacy of anticancer agents against multiple tumor cell lines in two physiological compartments of one animal. In this assay, semipermeable biocompatible fibers are used, which can be filled with cancer cells. These cancer cells can be derived from different tissue origins and can have different cellular characteristics. The fibers are made of polyvinylidene...


National Cancer Institute Tumor Cell Line Xenograft Model Hollow Fiber Relative Tumor Volume 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of Medical OncologyVU University Medical CenterAmsterdamThe Netherlands