Encyclopedia of Cancer

Living Edition
| Editors: Manfred Schwab

Flow Cytometry

  • Anurag Saxena
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27841-9_2209-2

Definition

FCM is a method for counting, analyzing, and sorting particles, e.g., cells in a single cell suspension by their light-absorbing or fluorescing properties. Flow cytometry permits assessment of physical (cell size, shape, and internal complexity), chemical (expression of proteins and other molecules), and biological (metabolic pathways, e.g., calcium ion flux) attributes of single cells in suspension (FACS).

Characteristics

  1. 1.

    The Technology

     
  2. 2.

    Applications in cancer

     

The Technology

There are three essential components of a flow cytometer: fluidics, optics, and electronics. Fluidics: A single cell suspension in a highly controlled fluid stream is forced through a nozzle to induced laminar flow at a high flow rate (meters/second). A higher-pressure sheath fluid stream surrounds the sample in isotonic fluid and the cells are examined (interrogated) at a point by the excitation light, usually a laser beam. Optics: A laser light source generates a monochromatic light beam...

Keywords

Chronic Lymphocytic Leukemia Minimal Residual Disease Minimal Residual Disease Assessment Fluorescence Resonance Energy Transfer Measurement Green Fluorescent Protein Label 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Cazzaniga G, Gaipa G, Rossi V et al (2006) Monitoring of minimal residual disease in leukemia, advantages and pitfalls. Ann Med 38:512–521CrossRefPubMedGoogle Scholar
  2. Cram LS (2002) Flow cytometry, an overview. Methods Cell Sci 24:1–9CrossRefPubMedGoogle Scholar
  3. Gudgin EJ, Erber WN (2005) Immunophenotyping of lymphoproliferative disorders: state of the art. Pathology 37:457–478CrossRefPubMedGoogle Scholar
  4. Kaleem Z (2006) Flow cytometric analysis of lymphomas: current status and usefulness. Arch Pathol Lab Med 130:1850–1858PubMedGoogle Scholar
  5. Megyeri A, Bacso Z, Shields A et al (2005) Development of a stereological method to measure levels of fluoropyrimidine metabolizing enzymes in tumor sections using laser scanning cytometry. Cytometry A 64:62–71CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of Pathology and Laboratory MedicineRoyal University Hospital, Saskatoon Health Region/University of SaskatchewanSaskatoonCanada