Early B-Cell Factors
Early B-cell factors are a group of DNA-binding transcription factors containing the helix-loop-helix (HLH) domain. Within their highly conserved DNA-binding domain (DBD), a sequence motif consisting of an atypical zinc finger (H-X3-C-X2-C-X5-C) is unique to this family of proteins.
Early B-cell factor 1 (EBF1) was initially isolated in 1993 from the nuclear extracts of a murine pre-B-cell line through oligonucleotide affinity chromatography. The DNA sequence of the oligonucleotide was derived from the promoter of the mb-1 gene, encoding an immunoglobulin-associated protein that is only expressed in the early stages of B-lymphocyte differentiation. EBF1 is essential for B-cell development, as mice lacking EBF1gene do not produce functional B cells and immunoglobulins. The cDNA encoding a related factor called Olf-1 was...