Doublecortex (DCX) is a brain-specific gene, deficient in glioma tumor cells and suppresses tumor in brain. DCX is a substrate of Jun N-terminal Kinase (JNK) and highly phosphorylated by JNK. The phosphorylated DCX interacts with a potent tumor suppressor, spinophilin neurabin II, and then associates with the protein serine/threonine phosphatase, protein phosphatase-1 (PP1). This association of protein complex of DCX, neurabin II, and PP1 leads to inhibition of anchorage-independent growth of glioma tumor cells and eventually suppresses tumor development.
DCX is a microtubule-associated protein(MAP), which directly interacts with microtubules (MTs) without any additional mediators. This interaction results in stabilization and bundling of MTs both in vivo and in vitro. DCX is a basic protein with an isoelectric point of 10, typical of other MT-binding proteins. DCX is an evolutionarily conserved MT binding protein...
KeywordsGlioma Cell Okadaic Acid Dynamic Instability Cell Adhesion Protein Common Genetic Alteration
- Santra M, Lui XS, Zhang J et al (2006b) Ectopic expression of doublecortin protects adult mouse progenitor cells and human glioma cells from severe oxygen and glucose deprivation. Neuroscience 92:7016–7020Google Scholar