Combinatorial Selection Methods

Synonyms

CASTing; In vitro genetics; REPSA; SAAB; SELEX; TDA

Definition

Combinatorial selection methods refer to a series of reiterative approaches involving large pools of randomized oligonucleotides, a selection process, and PCR amplification for identifying preferred ligand-binding sites on nucleic acid receptors.

Characteristics

Combinatorial selection methods are reiterative in vitro methods used to find the preferred nucleic acid-binding sequences of many ligand types. Examples of combinatorial selection methods include cyclic amplification and selection of targets (CASTing), in vitro genetics, restriction endonuclease protection, selection, and amplification (REPSA), and systematic evolution of ligands by exponential enrichment (SELEX). Typically these combinatorial methods involve large populations of nucleic acids containing a region of randomized sequence, a selection process, a means of amplifying the selected subpopulation, and the ability to cyclically repeat selection and...