Encyclopedia of Cancer

Living Edition
| Editors: Manfred Schwab

Combinatorial Selection Methods

  • Michael W. Van Dyke
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27841-9_1273-2

Synonyms

Definition

Combinatorial selection methods refer to a series of reiterative approaches involving large pools of randomized oligonucleotides, a selection process, and PCR amplification for identifying preferred ligand-binding sites on nucleic acid receptors.

Characteristics

Combinatorial selection methods are reiterative in vitro methods used to find the preferred nucleic acid-binding sequences of many ligand types. Examples of combinatorial selection methods include cyclic amplification and selection of targets (CASTing), in vitro genetics, restriction endonuclease protection, selection, and amplification (REPSA), and systematic evolution of ligands by exponential enrichment (SELEX). Typically these combinatorial methods involve large populations of nucleic acids containing a region of randomized sequence, a selection process, a means of amplifying the selected subpopulation, and the ability to cyclically repeat selection and...

Keywords

Polymerase Chain Reaction Amplification Direct Polymerase Chain Reaction Selection Template Affinity Species Natural Nucleic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Gold L, Polisky B, Uhlenbeck O et al (1995) Diversity of oligonucleotide functions. Annu Rev Biochem 64:763–797CrossRefPubMedGoogle Scholar
  2. Ouellette MM, Wright WE (1995) Use of reiterative selection for defining protein–nucleic acid interactions. Curr Opin Biotechnol 6:65–72CrossRefPubMedGoogle Scholar
  3. Szostak JW (1993) In vitro genetics. TIBS 17:89–93Google Scholar
  4. Van Dyke MW, Van Dyke N, Sunavala-Dossabhoy G (2007) REPSA: general combinatorial approach for identifying preferred ligand–DNA binding sequences. Methods 42:118–127CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryKennesaw State UniversityKennesawUSA