Chromophore-Assisted Laser Inactivation
Living reference work entry
Technology to address protein function in situ. CALI uses laser light of 620 nm, targeted via specific malachite green-labeled non-function-blocking antibodies, which generate short-lived protein-damaging free radicals (Fig. 1). This wavelength is not absorbed by cells, such that nonspecific light damage does not occur. The short lifetime of the free radicals generated restricts the damage largely to the bound antigen (∼15 Å) such that even neighboring proteins are not significantly affected. Micro-CALI focuses the laser light through microscope optics such that proteins within a 10 μ spot may be inactivated.
KeywordsHigh Resolution Mass Spectrometry Target Validation Functional Inactivation Myosin Versus Nerve Growth Cone
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