Encyclopedia of Astrobiology

Living Edition
| Editors: Muriel Gargaud, William M. Irvine, Ricardo Amils, Henderson James Cleaves, Daniele Pinti, José Cernicharo Quintanilla, Michel Viso

Ion-Exchange Chromatography

  • Mark Dörr
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27833-4_805-2

Synonyms

IEC

Definition

Ion-exchange chromatography (IEC) is a laboratory separation process for the separation of ions and polar molecules based on their charge. It can be used for a multitude of charged molecules, including large molecules, such as proteins and oligonucleotides, and small molecules, such as amino acids. The stationary phase commonly consists of a resin or gel with a covalently linked charged molecule. Depending on the charge, IEC can be either anion- or cation-exchange chromatography. It is often used for protein purification, DNA/RNA oligomer analysis, and water analysis.

History

Ion-exchange chromatography is one of the oldest separation processes described in literature. In 1850, H. Thompson and J. T. Way treated various clays (= stationary phase) with ammonium sulfate or carbonate in solution to release calcium. During World War II, ion-exchange chromatography was further developed and played a crucial role in the “Manhattan project” to enrich radioactive...

Keywords

Radioactive Element Stationary Phase Small Molecule Bioorganic Chemistry Affinity Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.University of Southern DenmarkOdense MDenmark