Encyclopedia of Astrobiology

Living Edition
| Editors: Muriel Gargaud, William M. Irvine, Ricardo Amils, Henderson James Cleaves, Daniele Pinti, José Cernicharo Quintanilla, Michel Viso

Electrophoresis

  • Adrienne Kish
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27833-4_500-2

Definition

Electrophoresis is the migration of charged molecules (for example nucleic acids or proteins) through a medium according to charge and molecular size as a consequence of an applied electric current and interactions with the medium. It is used as an analytical technique to separate molecules by charge, size, or binding affinity. Parameters including matrix composition and concentration, ionic strength, electrical current strength and field angle, and migration time can be varied. Electrophoretic methods commonly employed include capillary electrophoresis (CE), gel electrophoresis (GE), and pulsed-field gel electrophoresis (PFGE) for separation of chromosomal DNA; denaturing and/or temperature gradient gel electrophoresis (DGGE/TGGE) for separation according to sequence; polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE for separation of proteins in their native and denatured conformations, respectively; and isoelectric focusing (IEF) and 2D electrophoresis for...

Keywords

Nucleic Acid Capillary Electrophoresis Isoelectric Point Migration Time Matrix Composition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Institut de Génétique et MicrobiologieUniversite Paris-Sud 11Orsay CedexFrance