Living reference work entry
Chromatographic coelution occurs when two (or more) compounds do not chromatographically separate due to the fact that both species have retention times that differ by less than the resolution of the method. This can be solved by increasing the selectivity and/or efficiency of the chromatography or by employing different detection techniques that can differentiate the coeluting compounds. Changing the chemistry of the mobile phase, stationary phase, temperature, and column or plane length are good methods to increase the separation. Techniques such as mass spectrometry and optical spectroscopy are common ways to distinguish between coeluting compounds that cannot be resolved (Fig. 1).
KeywordsMass Spectrometry Mobile Phase Retention Time Stationary Phase Bioorganic Chemistry
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References and Further Reading
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