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The aim of the lymphocyte transformation test is to determine by in vitro methods whether a patient has developed a T-cell response against a certain drug. Such T-cell responses are present in practically all types of allergic reactions (type I–IV reactions), which means also in antibody-mediated reactions (Pichler and Tilch 2004). For that purpose, peripheral blood mononuclear cells obtained after density gradient centrifugation (Ficoll-Hypaque) are cultured with different concentrations of the incriminated drug for 5–6 days (Pichler and Tilch 2004; Nyfeler and Pichler 1997; Neukomm et al. 2001; Maria and Victorino 1997; Tsutsui et al. 1992; Naisbitt et al. 2003a, b). The drug concentration used in this in vitro assay has previously been determined by establishing toxic concentrations (e.g., by evaluating an inhibition on PHA stimulations). The proliferation of the...
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References
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© 2014 Springer-Verlag Berlin Heidelberg
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Pichler, W.J. (2014). Lymphocyte Transformation Test. In: Vohr, HW. (eds) Encyclopedia of Immunotoxicology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27786-3_924-5
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DOI: https://doi.org/10.1007/978-3-642-27786-3_924-5
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Publisher Name: Springer, Berlin, Heidelberg
Online ISBN: 978-3-642-27786-3
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