Synonyms
Brain microdialysis; Intracerebral microdialysis
Definition
Brain microdialysis is a sampling technique developed to study the concentration of chemicals (mainly neurotransmitters and their metabolites) in the extracellular compartment of the brain by means of implanting a small tubing equipped with a dialysis membrane. During the last decades, the necessity to measure the release of neurotransmitters in vivo in the central nervous system (CNS) has prompted the development of innovative techniques for sampling the extracellular fluid in the brain of experimental animals. Historically, one of the methods that evolved for this purpose was the push-pull perfusion which involved the stereotaxic insertion of a push-pull cannula into a selected area of the brain. Being an open flow system, push-pull perfusion allowed a direct contact of perfusion fluid with brain tissue, which often caused tissue damage, microbial and blood contamination, etc. To circumvent such drawbacks, a...
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References
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Adell, A., Artigas, F. (2013). Microdialysis. In: Stolerman, I., Price, L. (eds) Encyclopedia of Psychopharmacology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27772-6_299-2
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DOI: https://doi.org/10.1007/978-3-642-27772-6_299-2
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