Encyclopedia of Parasitology

Living Edition
| Editors: Heinz Mehlhorn

Drug Targets

  • Heinz Mehlhorn
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27769-6_4970-1

In recent times, various contemporary approaches to identify and characterize drug targets in helminth parasites have been developed: (a) rapid sequencing of parasite genomes using next-generation sequencing machines like 454 and Illumina to quickly spot and identify unique/divergent genes; (b) proteomic analysis (2D, mass spectrometry, SILAC, etc.) of the parasites or part of the parasites; (c) biophysical and biochemical characterization (X-ray, crystallography, UV-visible spectroscopy, circular dichroism spectroscopy, isothermal titration calorimetry, neurobiology, enzymology, etc.) of drug targets; (d) localization study and RNA interference of genes to know the essentiality and functions of unknown genes in nematode model organism called C. elegans; (e) in vitro screening of hits against drug targets in culture plates using MTT assays, NTS assay, etc.; and (f) in vivo screening of leads (obtained after multiple screening and selecting the best hits) in appropriate animal model...

Keywords

Mass Spectrometry Drug Target 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Further Reading

  1. Rana AK, Misra-Bhattacharya S (2013) Current drug targets for helminthic diseases. Parasitol Res 112:1819–1831CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Institut für Zoomorphologie, Zellbiologie und ParasitologieHeinrich-Heine-UniversitätDüsseldorfGermany