Encyclopedia of Parasitology

Living Edition
| Editors: Heinz Mehlhorn

Drug Targets

  • Heinz Mehlhorn
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27769-6_4970-1

In recent times, various contemporary approaches to identify and characterize drug targets in helminth parasites have been developed: (a) rapid sequencing of parasite genomes using next-generation sequencing machines like 454 and Illumina to quickly spot and identify unique/divergent genes; (b) proteomic analysis (2D, mass spectrometry, SILAC, etc.) of the parasites or part of the parasites; (c) biophysical and biochemical characterization (X-ray, crystallography, UV-visible spectroscopy, circular dichroism spectroscopy, isothermal titration calorimetry, neurobiology, enzymology, etc.) of drug targets; (d) localization study and RNA interference of genes to know the essentiality and functions of unknown genes in nematode model organism called C. elegans; (e) in vitro screening of hits against drug targets in culture plates using MTT assays, NTS assay, etc.; and (f) in vivo screening of leads (obtained after multiple screening and selecting the best hits) in appropriate animal model...


Mass Spectrometry Drug Target 
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Further Reading

  1. Rana AK, Misra-Bhattacharya S (2013) Current drug targets for helminthic diseases. Parasitol Res 112:1819–1831CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Institut für Zoomorphologie, Zellbiologie und ParasitologieHeinrich-Heine-UniversitätDüsseldorfGermany