Encyclopedia of Parasitology

Living Edition
| Editors: Heinz Mehlhorn

Trichuris suis Artificial Larval Hatching

  • Heinz Mehlhorn
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27769-6_4469-1
The larvae of Trichuris suis – whipworm of pigs – can be used to stimulate the immune system of humans and thus block for up to 3 months of autoimmune reactions which lead to symptoms such as Morbus Crohn or ulcerative colitis. In cases of treatment, about 2,000 larvae-containing eggs have to be swallowed within some milliliter of water. In order to control, whether a sufficient number of larvae hatched and remained motile inside the intestine, a so-called hatch test must be done from time to time with stored eggs. By the way the larvae of T. suis do not reach maturity in humans, but die within 3 months after hatch. The process needs the following steps:
  1. 1.

    Incubation of the eggs in a 13 % hypochlorite solution at 40 °C and 5 % CO2 for 2–5 h

  2. 2.

    Washing and centrifugation (1,000 rpm) within a Trichuris-hatching medium (see below)

  3. 3.

    Treatment with ultrasonic waves for 1 min

  4. 4.

    Transfer of treated eggs into plastic wells with hatching medium at 37–40 °C

  5. 5.


Immune System Ulcerative Colitis Fetal Calf Serum Calf Serum Ultrasonic Wave 
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Further Reading

  1. Bager P et al (2011) Symptoms after ingestion of pig whipworm Trichuris suis eggs in a randomized placebo-controlled double-blind clinical trial. PLoS ONE 6(8):e22346CrossRefPubMedPubMedCentralGoogle Scholar
  2. Garud S, Peppercorn MA (2009) Ulcerative colitis: current treatment strategies and future prospects. Therap Adv Gastroenterol 2:99–108CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Institut für Zoomorphologie, Zellbiologie und ParasitologieHeinrich-Heine-UniversitätDüsseldorfGermany