Encyclopedia of Parasitology

Living Edition
| Editors: Heinz Mehlhorn

SAFC Concentration

  • Heinz Mehlhorn
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27769-6_4310-1
This is a method to isolate parasitic stages from feces respectively stools of animals or humans by help of a so-called sodium acetate-acetic acid-formalin solution.
  1. 1.

    Stool sample (1 g) is placed into a tube containing 10 ml of S.A.F.

     
  2. 2.

    Stool and S.A.F. are intensively mixed by a glass stick.

     
  3. 3.

    Closed tube is shaken vigorously.

     
  4. 4.

    Stool is filtered using a funnel with gauze.

     
  5. 5.

    The tubes are now centrifuged for 1 minute at 2,000 rpm.

     
  6. 6.

    Supernatant is removed by help of a pipette or cautiously decanted.

     
  7. 7.

    7 ml saline is added and contents are mixed with a glass stick.

     
  8. 8.

    3 ml ether has to be added.

     
  9. 9.

    Tubes are closed with rubber stoppers, well shaken, while fixing firmly the stopper (or closing the vessel by a cover).

     
  10. 10.

    The cover (or rubber stopper) is removed and the product is centrifuged for 5 min at 2,000 rpm.

     
  11. 11.

    After centrifugation all three upper layers were decanted and just the sediment is retained and investigated by help of a light microscope...

Keywords

Light Microscope Stool Sample Cover Slip Rubber Stopper Parasitic Stage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Further Reading

  1. Blagg W et al (1955) New concentration technique for demonstration of protozoan and helminth eggs in faeces. Am J Trop Meg Hyg 4:23–28Google Scholar
  2. Marti H, Escher E (1980) SAF – an alternative fixation solution for parasitological stool specimens. Schweiz Med Wochenschr 120:1473–1476Google Scholar
  3. Mehlhorn H et al (1992) Diagnose und Therapie der Parasiten von Haus-, Nutz- und Heimtieren, 2nd edn. Gustav Fischer Verlag, Stuttgart/New YorkGoogle Scholar
  4. Price LD (1981) Comparison of three collection-preservation methods for detection of intestinal parasites. J Clin Microbiol 14:656–660PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Institut für Zoomorphologie, Zellbiologie und ParasitologieHeinrich-Heine-UniversitätDüsseldorfGermany