Stool sample (1 g) is placed into a tube containing 10 ml of S.A.F.
Stool and S.A.F. are intensively mixed by a glass stick.
Closed tube is shaken vigorously.
Stool is filtered using a funnel with gauze.
The tubes are now centrifuged for 1 minute at 2,000 rpm.
Supernatant is removed by help of a pipette or cautiously decanted.
7 ml saline is added and contents are mixed with a glass stick.
3 ml ether has to be added.
Tubes are closed with rubber stoppers, well shaken, while fixing firmly the stopper (or closing the vessel by a cover).
The cover (or rubber stopper) is removed and the product is centrifuged for 5 min at 2,000 rpm.
After centrifugation all three upper layers were decanted and just the sediment is retained and investigated by help of a light microscope...
KeywordsLight Microscope Stool Sample Cover Slip Rubber Stopper Parasitic Stage
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