Encyclopedia of Parasitology

Living Edition
| Editors: Heinz Mehlhorn

Negative Staining

  • Heinz Mehlhorn
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27769-6_4092-1
This term describes a method to be used as well in light as in transmission electron microscopy. A thin, tiny specimen is covered with an optically opaque fluid (e.g., black ink etc.) thus staining the background and leaving visible the cell/parasite, etc. In case of positive staining, the target itself would be stained. In the case of transmission electron microscopy, specimens are stained, for example, with uranyl acetate or phosphotungstic acid. Note: During this slow process, living infectious organisms remain infectious for a while. Thus an infection risk exists (Fig. 1).

Keywords

Electron Microscopy Transmission Electron Microscopy Positive Staining Uranyl Acetate Slow Process 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Further Reading

  1. Hayat MA (2000) Principles and techniques for electron microscopy. Cambridge University Press, CambridgeGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Institut für Zoomorphologie, Zellbiologie und ParasitologieHeinrich-Heine-UniversitätDüsseldorfGermany