Encyclopedia of Parasitology

Living Edition
| Editors: Heinz Mehlhorn

Negative Staining

  • Heinz Mehlhorn
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-27769-6_4092-1
This term describes a method to be used as well in light as in transmission electron microscopy. A thin, tiny specimen is covered with an optically opaque fluid (e.g., black ink etc.) thus staining the background and leaving visible the cell/parasite, etc. In case of positive staining, the target itself would be stained. In the case of transmission electron microscopy, specimens are stained, for example, with uranyl acetate or phosphotungstic acid. Note: During this slow process, living infectious organisms remain infectious for a while. Thus an infection risk exists (Fig. 1).


Electron Microscopy Transmission Electron Microscopy Positive Staining Uranyl Acetate Slow Process 
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Further Reading

  1. Hayat MA (2000) Principles and techniques for electron microscopy. Cambridge University Press, CambridgeGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Institut für Zoomorphologie, Zellbiologie und ParasitologieHeinrich-Heine-UniversitätDüsseldorfGermany