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Microfluidics for Studies of Apoptosis

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Encyclopedia of Microfluidics and Nanofluidics

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Synonyms

Apoptosis; Caspase activation; Cell membrane changes; Changes of caspase activity; DNA fragmentation; DNA ladders; Externalization of phosphatidylserine; Flip of phosphatidylserine; Internucleosomal DNA fragmentation; Intracellular Ca2+ concentration changes; Intracellular Ca2+ fluxes; Lab-on-a-chip; Micro total analysis system (μTAS); Microfluidics; Programmed cell death; Surface lipid translocation; Translocation of phosphatidylserine; μTAS

Definition

Microfluidic systems, lab-on-a-chip systems, or micro total analysis systems (μTAS) allow the miniaturization of various functional units, such as pumps, valves, and reactors, and make it possible to build novel integrated microsystems for various biological applications. These systems have been well recognized as a possible enabling technologythat may play important roles in biology in the future. This observation is supported by the efforts that have been made to build functional microsystems for various studies of cells,...

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References

  1. Manz A, Graber N, Widmer HM (1990) Miniaturized total chemical analysis systems: a novel concept for chemical sensing. Sens Actuators B 1:244–248

    Article  Google Scholar 

  2. Andersson H, van den Berg A (2003) Microfluidic devices for cellomics: a review. Sens Actuators B 92:315–325

    Article  Google Scholar 

  3. Takayama S et al (2001) Laminar flows – subcellular positioning of small molecules. Nature 411:1016

    Article  Google Scholar 

  4. Lucchetta EM, Lee JH, Fu LA, Patel NH, Ismagilov RF (2005) Dynamics of Drosophila embryonic patterning network perturbed in space and time using microfluidics. Nature 434:1134–1138

    Article  Google Scholar 

  5. Lu H, Gaudet S, Schmidt MA, Jensen KF (2004) A microfabricated device for subcellular organelle sorting. Anal Chem 76:5705–5712

    Article  Google Scholar 

  6. Buhlmann C, Valer M, Mueller O (2004) Combination of DNA laddering and annexin-V and caspase assays on one system – multiple apoptosis parameters analysis with a microfluidic chip-based system. Tissue Antigens 64:392–392

    Google Scholar 

  7. Chan SDH, Luedke G, Valer M, Buhlmann C, Preckel T (2003) Cytometric analysis of protein expression and apoptosis in human primary cells with a novel microfluidic chip-based system. Cytometry 55(A):119–125

    Article  Google Scholar 

  8. Qin JH et al (2005) Simultaneous and ultrarapid determination of reactive oxygen species and reduced glutathione in apoptotic leukemia cells by microchip electrophoresis. Electrophoresis 26:1155–1162

    Article  Google Scholar 

  9. Kleparnik K, Horky M (2003) Detection of DNA fragmentation in a single apoptotic cardiomyocyte by electrophoresis on a microfluidic device. Electrophoresis 24:3778–3783

    Article  Google Scholar 

  10. Sohn LL et al (2000) Capacitance cytometry: measuring biological cells one by one. Proc Natl Acad Sci U S A 97:10687–10690

    Article  Google Scholar 

  11. Tabuchi M, Baba Y (2004) Self-contained on-chip cell culture and pretreatment system. J Proteome Res 3:871–877

    Article  Google Scholar 

  12. Tamaki E et al (2002) Single-cell analysis by a scanning thermal lens microscope with a microchip: direct monitoring of cytochrome c distribution during apoptosis process. Anal Chem 74:1560–1564

    Article  Google Scholar 

  13. Wheeler AR et al (2003) Microfluidic device for single-cell analysis. Anal Chem 75:3581–3586

    Article  Google Scholar 

  14. Yang MS, Li CW, Yang J (2002) Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device. Anal Chem 74:3991–4001

    Article  Google Scholar 

  15. Andersson H, van den Berg A (2004) Microtechnologies and nanotechnologies for single-cell analysis. Curr Opin Biotechnol 15:44–49

    Article  Google Scholar 

  16. Hong JW, Studer V, Hang G, Anderson WF, Quake SR (2004) A nanoliter-scale nucleic acid processor with parallel architecture. Nat Biotechnol 22:435–439

    Article  Google Scholar 

  17. Muñoz-Pinedo C, Green DR, van den Berg A (2005) Confocal restricted-height imaging of suspension cells (CRISC) in a PDMS microdevice during apoptosis. Lab Chip 5:628–633

    Article  Google Scholar 

  18. Valero A et al (2005) Apoptotic cell death dynamics of HL60 cells studied using a microfluidic cell trap device. Lab Chip 5:49–55

    Article  Google Scholar 

  19. Ye NN, Qin JH, Shi WW, Liu X, Lin BC (2007) Cell-based high content screening using an integrated microfluidic device. Lab Chip 7:1696–1704

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biotechnology Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China

    Jianhua Qin & Nannan Ye

  2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China

    Bingcheng Lin

Authors
  1. Jianhua Qin
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  2. Nannan Ye
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  3. Bingcheng Lin
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Corresponding author

Correspondence to Jianhua Qin .

Editor information

Editors and Affiliations

  1. VU Station B 351592, Vanderbilt University Dept. Mechanical Engineering, Nashville, Tennessee, USA

    Dongqing Li

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© 2014 Springer Science+Business Media New York

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Qin, J., Ye, N., Lin, B. (2014). Microfluidics for Studies of Apoptosis. In: Li, D. (eds) Encyclopedia of Microfluidics and Nanofluidics. Springer, Boston, MA. https://doi.org/10.1007/978-3-642-27758-0_953-2

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  • DOI: https://doi.org/10.1007/978-3-642-27758-0_953-2

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  • Publisher Name: Springer, Boston, MA

  • Online ISBN: 978-3-642-27758-0

  • eBook Packages: Springer Reference EngineeringReference Module Computer Science and Engineering

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