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Chemiluminescence Detection

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Encyclopedia of Microfluidics and Nanofluidics
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Chemical luminescence detection


Chemiluminescence is the emission of light from excited molecules, ions, or atoms formed in a chemical reaction. As the intensity of emitted light is dependent on the number of reacting molecules and can be measured against a dark background, this phenomenon provides a simple and highly sensitive means for chemical detection.

Chemical and Physical Principles

Luminescence is the emission of light from excited molecules, ions, or atoms as they relax back to their ground state, which can be further categorized by the mode of excitation. For example, in “photoluminescence,” such as the fluorescence of tonic water, the emitting species is excited by the absorption of light, whereas “triboluminescence” arises from the structural rearrangements of solids, such as when sucrose is crushed or wintergreen Life Savers candy are broken.

In “chemiluminescence,” the excited states are attained from the excess energy of chemical reactions. The most...

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Further Reading and References

  1. Mirasoli M, Guardiglia M, Michelini E, Roda A (2013) Recent advancements in chemical luminescence-based lab-on-chip and microfluidic platforms for bioanalysis. J Pharm Biomed Anal 87:36–52

    Article  Google Scholar 

  2. Kricka LJ, Park JY (2011) Miniaturized analytical devices based on chemiluminescence, bioluminescence and electrochemiluminescence. In: Roda A (ed) Chemiluminescence and bioluminescence: past, present and future. RSC, Cambridge, pp 543–556

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  3. Roda A, Mirasoli M, Roda B, Reschiglian P (2011) Flow-assisted analysis. In: Roda A (ed) Chemiluminescence and bioluminescence: past, present and future. RSC, Cambridge, pp 191–226

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  4. Gai H, Li Y, Yeung ES (2011) Optical detection systems on microfluidic chips. In: Lin B (ed) Microfluidics: technologies and applications, vol 304, Topics in current chemistry. Springer, Heidelberg, pp 171–201

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  5. García-Campaña AM, Baeyens WRG (eds) (2001) Chemiluminescence in analytical chemistry. Marcel Dekker, New York

    Google Scholar 

  6. Adcock JL, Barrow CJ, Barnett NW, Conlan XA, Hogan CF, Francis PS (2011) Chemiluminescence and electrochemiluminescence detection of controlled drugs. Drug Test Anal 3:145–160

    Article  Google Scholar 

  7. Al Lawati HAJ (2013) Flow-based analysis using microfluidics-chemiluminescence systems. Luminescence 28:618–627

    Article  Google Scholar 

  8. Kricka LJ (2003) Optical methods: a guide to the “-escences”. AACC Press, Washington, DC, p 191

    Google Scholar 

  9. Francis PS, Adcock JL (2012) Liquid-phase chemiluminescence detection for HPLC. In: Andrew Shalliker R (ed) Hyphenated and alternative methods of detection in chromatography. Chromatographic science series, CRC Press, Boca Raton, Florida, vol 104. pp 221–250

    Google Scholar 

  10. Huang X, Ren J (2006) Chemiluminescence detection for capillary electrophoresis and microchip capillary electrophoresis. Trends Anal Chem 25(2):155–166

    Article  MathSciNet  Google Scholar 

  11. Terry JM, Mohr S, Fielden PR, Goddard NJ, Barnett NW, Olson DC, Wolcott DK, Francis PS (2012) Chemiluminescence detection flow-cells for flow injection analysis and high performance liquid chromatography. Anal Bioanal Chem 403:2353–2360

    Article  Google Scholar 

  12. Al Lawati HAJ, Suliman FEO, Al Kindy SMZ, Al-Lawati AM, Varma GB, Nour IEM (2010) Enhancement of on chip chemiluminescence signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate in pharmaceutical formulations. Talanta 82(5):1999–2002

    Article  Google Scholar 

  13. Tsukagoshi K, Jinno N, Nakajima R (2005) Development of a micro total analysis system incorporating chemiluminescence detection and application to detection of cancer markers. Anal Chem 77(6):1684–1688

    Article  Google Scholar 

  14. Ye F, Huang Y, Xu Q, Shi M, Zhao S (2010) Quantification of taurine and amino acids in mice single fibrosarcoma cell by microchip electrophoresis coupled with chemiluminescence detection. Electrophoresis 31(10):1630–1636

    Article  Google Scholar 

  15. Wang S, Ge L, Song X, Yu J, Ge S, Huang J, Zeng F (2012) Paper-based chemiluminescence ELISA: lab-on-paper based on chitosan modified paper device and wax-screen-printing. Biosens Bioelectron 31(1):212–218

    Article  Google Scholar 

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Correspondence to Paul S. Francis .

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Francis, P.S. (2014). Chemiluminescence Detection. In: Li, D. (eds) Encyclopedia of Microfluidics and Nanofluidics. Springer, Boston, MA.

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