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Cell Sorting

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Encyclopedia of Microfluidics and Nanofluidics

Synonyms

Cell fractionation; Cell separation

Definition

Cell sorting is defined as the separation of a heterogeneous mixture of cells into purified fractions of homogeneous cells. In this context it will also include the isolation of individual cell types from a heterogeneous mixture of cells. In this context cell refers to prokaryotic and eukaryotic cells.

Overview

Methods for processing microliter samples containing cells are in high demand for a wide range of applications in areas such as biomedical research, clinical diagnostics, counterterrorism, environmental analysis, and biotechnology. Development of a cell-based screening assay often requires identification and isolation of particular cells from a mixture of various kinds of cells in various stages of development. Moreover, in order to obtain reproducible data on cells, reliable and nondestructive purification of cells is essential. However, the efficient separation of cells is a nontrivial task. In addition to the typical...

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Correspondence to Louise M. Barrett .

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© 2014 Springer Science+Business Media New York

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Barrett, L.M., Simmons, B.A. (2014). Cell Sorting. In: Li, D. (eds) Encyclopedia of Microfluidics and Nanofluidics. Springer, Boston, MA. https://doi.org/10.1007/978-3-642-27758-0_199-2

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  • DOI: https://doi.org/10.1007/978-3-642-27758-0_199-2

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  • Online ISBN: 978-3-642-27758-0

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