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Cell Assays in Microfluidics

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Encyclopedia of Microfluidics and Nanofluidics

Synonyms

Cell analysis/testing in microfluidic devices; Cell assays/analysis/testing on chip; Phenotypic screening in microfluidic devices/on chip

Definition

A cell assay is defined as measurement and analysis of cellular response to chemical and/or physical stimulus. Cellular responses are diverse: alterations of intracellular and extracellular biochemistry, cell morphology, motility, and growth properties. These responses characterize the cell phenotype and are typically monitored in a culture dish or a multiwell plate, while more recently microfluidic devices have been employed. A cell assay performed in a microfluidic device is sometimes termed an on-chip assay.

High-throughput screening (HTS) is a class of analytical techniques in which many different assays are performed in parallel or very rapidly in succession. Currently, there is great interest in performing cell assays in an HTS format.

High-content screening (HCS) is a cell assay method in which phenotypic changes in live...

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Authors and Affiliations

  1. Kapplex, Inc., Suite 200 – 101 College Street, Toronto, ON, M5G 1L7, Canada

    Irena Barbulovic-Nad

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  1. Irena Barbulovic-Nad
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Correspondence to Irena Barbulovic-Nad .

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Editors and Affiliations

  1. VU Station B 351592, Vanderbilt University Dept. Mechanical Engineering, Nashville, Tennessee, USA

    Dongqing Li

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© 2014 Springer Science+Business Media New York

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Barbulovic-Nad, I. (2014). Cell Assays in Microfluidics. In: Li, D. (eds) Encyclopedia of Microfluidics and Nanofluidics. Springer, Boston, MA. https://doi.org/10.1007/978-3-642-27758-0_183-2

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  • DOI: https://doi.org/10.1007/978-3-642-27758-0_183-2

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  • Publisher Name: Springer, Boston, MA

  • Online ISBN: 978-3-642-27758-0

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