Absorption: In Vitro Tests – Non Cell Based

  • Markus Kohlmann
Reference work entry


The ability to permeate a biological membrane is a prerequisite for oral administered drugs. The primary absorption pathway for the majority of drugs after oral dosage still seems to be the passive transcellular diffusion pathway. A number of methods used in pharmaceutical research to early on estimate the passive absorption potential of a compound by means of a variety of cell free techniques like reversed phase HPLC, artificial membranes or liposomes and PAMPA are described.


Artificial Membrane Unstirred Water Layer Parallel Artificial Membrane Permeation Assay Membrane Affinity Immobilize Artificial Membrane 
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References and Further Reading

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© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Sanofi Deutschland GmbHFrankfurt am MainGermany

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