Synonyms
Definition
High-speed AFM (HS-AFM) is a type of atomic force microscopy (AFM) that, unlike conventional AFM, can take an image very quickly and with relatively low imaging force, therefore allowing for visualizing the dynamic behavior of biomolecules.
Introduction
AFM can take high-resolution images of objects in various environments. In particular, its in-liquid imaging capability is useful for biological research. However, the imaging rate of conventional AFM is too low to capture dynamic processes of biomolecules (DNA, proteins). HS-AFM has been developed to overcome this problem by optimizing all devices contained in the instrument for fast scanning and by newly developing fast and precise control techniques (Ando et al. 2001, 2008). The imaging rate of the current HS-AFM has reached 15–25 frames per second (fps) for a scan range of ∼240×240 nm2with 100 scan lines. By further reducing the scan range, the imaging rate can exceed 50 fps....
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ando T, Kodera N, Takai E, Maruyama D, Saito K, Toda A. A high-speed atomic force microscope for studying biological macromolecules. Proc Natl Acad Sci USA. 2001;98:12468–72.
Ando T, Uchihashi T, Fukuma T. High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes. Prog Surf Sci. 2008;83:337–437.
Giocondi MC, Yamamoto D, Lesniewska E, Milhiet PE, Ando T, Le Grimellec C. Surface topography of membrane domains. Biochim Biophys Acta -Biomembranes 2010;1798:703–18.
Kodera N, Sakashita M, Ando T. Dynamic proportional-integral-differential controller for high-speed atomic force microscopy. Rev Sci Instrum. 2006;77:083704 (7 pp).
Kodera N, Yamamoto D, Ishikawa R, Ando T. Video imaging of walking myosin V by high-speed atomic force microscopy. Nature. 2010;468:72–6.
Milhiet PE, Yamamoto D, Berthoumieu O, Dosset P, Le Grimellec C, Verdier JM, Marchal S, Ando T. Deciphering the structure, growth and assembly of amyloid-like fibrils using high-speed atomic force microscopy. PLoS One. 2010;5:e13240 (8Â pp).
Shibata M, Yamashita H, Uchihashi T, Kandori H, Ando T. High-speed atomic force microscopy shows dynamic molecular processes in photo-activated bacteriorhodopsin. Nat Nanotechnol. 2010;5:208–12.
Uchihashi T, Iino R, Ando T, Noji H. High-speed atomic force microscopy reveals rotary catalysis of rotor-less F1-ATPase. Science 2011;333:755–8.
Yamamoto D, Uchihashi T, Kodera N, Ando T. Anisotropic diffusion of point defects in two-dimensional crystal of streptavidin observed by high-speed atomic force microscopy. Nanotechnology. 2008;19:384009 (9Â pp).
Yamamoto D, Uchihashi T, Kodera N, Yamashita H, Nishikori S, Ogura T, Shibata M, Ando T. High-speed atomic force microscopy techniques for observing dynamic biomolecular processes. Methods Enzymol. 2010;475(B):541–64.
Yamashita H, Voïtchovsky K, Uchihashi T, Antoranz Contera S, Ryan JF, Ando T. Dynamics of bacteriorhodopsin 2D crystal observed by high-speed atomic force microscopy. J Struct Biol. 2009;167:153–8.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 European Biophysical Societies' Association (EBSA)
About this entry
Cite this entry
Ando, T. (2013). High-Speed Atomic Force Microscopy (AFM). In: Roberts, G.C.K. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16712-6_478
Download citation
DOI: https://doi.org/10.1007/978-3-642-16712-6_478
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-16711-9
Online ISBN: 978-3-642-16712-6
eBook Packages: Biomedical and Life SciencesReference Module Biomedical and Life Sciences