Surface-Enhanced Laser Desorption/Ionization Time of Flight Mass Spectrometry
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SELDI-TOF MS is an approach that combines two powerful technologies, retentate chromatography and MS. The core of the SELDI-TOF MS platform is the proteinchip arrays, which have varying chromatographic properties, such as anion exchange, cation exchange, metal affinity, and reverse phase. The SELDI-TOF MS provides on-chip separation as well as the capability to perform enzymatic reactions directly on the chip. Various complicated biological materials can be uniformly captured, concentrated, and purified on the small chemical surface of the chip. To be able to identify the proteins, the complexity of the sample was reduced by fractionation approaches. A complex mixture of proteins from cells or body fluids can be reduced to sets of proteins with common properties by binding the sample to chips with differing surface chemistries in parallel and in series.