Abstract:
Microautoradioactivity (MAR) in combination with fluorescence in situ hybridization (FISH) is a powerful method to obtain information about the ecophysiology of probe-defined single cells in mixed microbial communities. Here we give an overview of how to use MAR-FISH in various ecosystems and provide a detailed protocol for MAR-FISH, which includes sampling, incubation with radiotracers, the MAR procedure in combination with FISH and other staining techniques, microscopy, and trouble shooting.
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Nielsen, J.L., Nielsen, P.H. (2010). Combined Microautoradiography and Fluorescence in situ Hybridization (MAR-FISH) for the Identification of Metabolically Active Microorganisms. In: Timmis, K.N. (eds) Handbook of Hydrocarbon and Lipid Microbiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77587-4_319
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DOI: https://doi.org/10.1007/978-3-540-77587-4_319
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