Synonyms
Definition
Somatic cell nuclear transfer (SCNT) is a technique which transfers the nucleus of a donor somatic cell to the cytoplasm of an oocyte with its own nucleus removed. The somatic nucleus is reprogrammed in the oocyte, in which it contains the entire genomic information of the somatic cell and progressively develops into the blastocyst. Successive development of the blastocyst with proper stimuli can result in generation of cloned animals in reproductive cloning or embryonic stem cells (ESC) for cloning specific tissues and cell types.
Overview
Somatic cell nuclear transfer is a classical technique in biotechnology for reprogramming the nucleus of a somatic cell (Singh et al. 2019). The cell can resume pluripotency and further differentiate from the embryonic state into specific tissues and cell types that inherited the original genotype in the donor nucleus. With the embryotic cell being grown in a surrogate mother, a...
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References
Blackburn EH et al (2006) Telomeres and telomerase: the path from maize, Tetrahymena and yeast to human cancer and aging. Nat Med 12:1133–1138. https://doi.org/10.1038/nm1006-1133
Blasco MA (2002) Immunosenescence phenotypes in the telomerase knockout mouse. Springer Semin Immunopathol 24:75–85. https://doi.org/10.1007/s00281-001-0096-1
Bondioli KR (2018) Cloning of livestock by somatic cell nuclear transfer. In: Niemann H, Wrenzycki C (eds) Animal biotechnology 2: emerging breeding technologies. Springer International Publishing, Cham, pp 1–20. https://doi.org/10.1007/978-3-319-92348-2_1
Boonekamp JJ et al (2013) Telomere length behaves as biomarker of somatic redundancy rather than biological age. Aging Cell 12:330–332. https://doi.org/10.1111/acel.12050
Briggs R, King TJ (1952) Transplantation of living nuclei from blastula cells into enucleated frogs’ eggs. Proc Natl Acad Sci U S A 38:455–463. https://doi.org/10.1073/pnas.38.5.455
Campbell KH et al (1996) Sheep cloned by nuclear transfer from a cultured cell line. Nature 380:64–66. https://doi.org/10.1038/380064a0
Chung YG et al (2015) Histone demethylase expression enhances human somatic cell nuclear transfer efficiency and promotes derivation of pluripotent stem cells. Cell Stem Cell 17:758–766. https://doi.org/10.1016/j.stem.2015.10.001
Cibelli JB et al (1998) Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells. Nat Biotechnol 16:642–646. https://doi.org/10.1038/nbt0798-642
Cyranoski D (2018) First monkeys cloned with technique that made Dolly the sheep. Nature 553:387–388. https://doi.org/10.1038/d41586-018-01027-z
D’Amour KA et al (2006) Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 24:1392–1401. https://doi.org/10.1038/nbt1259
French AJ et al (2006) Human therapeutic cloning (NTSC): applying research from mammalian reproductive cloning. Stem Cell Rev 2:265–276. https://doi.org/10.1007/BF02698053
Guarente L (2007) Sirtuins in aging and disease. Cold Spring Harb Symp Quant Biol 72:483–488. https://doi.org/10.1101/sqb.2007.72.024
Gurdon JB (1962) The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. J Embryol Exp Morphol 10:622–640
Gurdon JB, Byrne JA (2003) The first half-century of nuclear transplantation. Proc Natl Acad Sci U S A 100:8048–8052. https://doi.org/10.1073/pnas.1337135100
Gurdon JB et al (1958) Sexually mature individuals of Xenopus laevis from the transplantation of single somatic nuclei. Nature 182:64–65. https://doi.org/10.1038/182064a0
Jaskelioff M et al (2011) Telomerase reactivation reverses tissue degeneration in aged telomerase-deficient mice. Nature 469:102–106. https://doi.org/10.1038/nature09603
Karlseder J et al (2002) Senescence induced by altered telomere state, not telomere loss. Science 295:2446–2449. https://doi.org/10.1126/science.1069523
Kawase E et al (2000) Mouse embryonic stem (ES) cell lines established from neuronal cell-derived cloned blastocysts. Genesis 28:156–163. https://doi.org/10.1016/j.reprotox.2018.07.080
Kfoury C (2007) Therapeutic cloning: promises and issues. Mcgill J Med 10:112–120
Kwon D et al (2017) Reprogramming enhancers in somatic cell nuclear transfer, iPSC technology, and direct conversion. Stem Cell Rev 13:24–34. https://doi.org/10.1007/s12015-016-9697-x
Lanza RP et al (1999a) Human therapeutic cloning. Nat Med 5:975–977. https://doi.org/10.1038/12404
Lanza RP et al (1999b) Prospects for the use of nuclear transfer in human transplantation. Nat Biotechnol 17:1171–1174. https://doi.org/10.1038/70709
Liu GH et al (2011) Recapitulation of premature ageing with iPSCs from Hutchinson-Gilford progeria syndrome. Nature 472:221–225. https://doi.org/10.1038/nature09879
Liu Z et al (2018) Cloning of macaque monkeys by somatic cell nuclear transfer. Cell 174:245. https://doi.org/10.1016/j.cell.2018.01.036
Lopez-Otin C et al (2013) The hallmarks of aging. Cell 153:1194–1217. https://doi.org/10.1016/j.cell.2013.05.039
Matoba S, Zhang Y (2018) Somatic cell nuclear transfer reprogramming: mechanisms and applications. Cell Stem Cell 23:471–485. https://doi.org/10.1016/j.stem.2018.06.018
Munsie MJ et al (2000) Isolation of pluripotent embryonic stem cells from reprogrammed adult mouse somatic cell nuclei. Curr Biol 10:989–992. https://doi.org/10.1016/s0960-9822(00)00648-5
Ocampo A et al (2016) Anti-aging strategies based on cellular reprogramming. Trends Mol Med 22:725–738. https://doi.org/10.1016/j.molmed.2016.06.005
Schaetzlein S et al (2004) Telomere length is reset during early mammalian embryogenesis. Proc Natl Acad Sci U S A 101:8034–8038. https://doi.org/10.1073/pnas.0402400101
Shay JW, Wright WE (2000) Hayflick, his limit, and cellular ageing. Nat Rev Mol Cell Biol 1:72–76. https://doi.org/10.1038/35036093
Shiels PG et al (1999) Analysis of telomere lengths in cloned sheep. Nature 399:316–317. https://doi.org/10.1038/20580
Sinclair KD et al (2016) Healthy ageing of cloned sheep. Nat Commun 7:12359. https://doi.org/10.1038/ncomms12359
Singh B et al (2019) Somatic cell nuclear transfer. In: Advances in animal biotechnology. Springer, Cham, pp 109–122. https://doi.org/10.1007/978-3-030-21309-1_10
Spemann H (1938) Embryonic development and induction. Yale University Press/H. Milford/Oxford University Press, London/New Haven
Sullivan EJ et al (2004) Cloned calves from chromatin remodeled in vitro. Biol Reprod 70:146–153. https://doi.org/10.1095/biolreprod.103.021220
Tabar V et al (2008) Therapeutic cloning in individual parkinsonian mice. Nat Med 14:379–381. https://doi.org/10.1038/nm1732
Tachibana M et al (2013) Human embryonic stem cells derived by somatic cell nuclear transfer. Cell 153:1228–1238. https://doi.org/10.1016/j.cell.2013.05.006
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676. https://doi.org/10.1016/j.cell.2006.07.024
Takahashi K et al (2007a) Induction of pluripotent stem cells from fibroblast cultures. Nat Protoc 2:3081–3089. https://doi.org/10.1038/nprot.2007.418
Takahashi K et al (2007b) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872. https://doi.org/10.1016/j.cell.2007.11.019
Thomson JA et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145–1147. https://doi.org/10.1126/science.282.5391.1145
Wakayama T et al (2000) Cloning of mice to six generations. Nature 407:318–319. https://doi.org/10.1038/35030301
Wilmut I et al (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385:810–813. https://doi.org/10.1038/385810a0
Wilmut I et al (2015) Somatic cell nuclear transfer: origins, the present position and future opportunities. Philos Trans R Soc Lond Ser B Biol Sci 370:20140366. https://doi.org/10.1098/rstb.2014.0366
Wolf DP et al (2017) Concise review: embryonic stem cells derived by somatic cell nuclear transfer: a horse in the race? Stem Cells 35:26–34. https://doi.org/10.1002/stem.2496
Zhang S et al (2016) Aberrant DNA methylation reprogramming in bovine SCNT preimplantation embryos. Sci Rep 6:30345. https://doi.org/10.1038/srep30345
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Tsang, P.Y.L., Tai, D.M.S., Li, L.C.W. (2020). Somatic Cell Nuclear Transfer. In: Gu, D., Dupre, M. (eds) Encyclopedia of Gerontology and Population Aging. Springer, Cham. https://doi.org/10.1007/978-3-319-69892-2_1043-1
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DOI: https://doi.org/10.1007/978-3-319-69892-2_1043-1
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