Synonyms
Tif5p (in yeast)
Introduction
The translation initiation process is mediated by a series of partial reactions each of which requires the participation of a large number of protein factors collectively called eukaryotic (translation) initiation factors (eIFs). The process leads to the assembly of an 80S ribosome-bound initiator Met-tRNAi at the start AUG codon of an mRNA that is active in peptidyl transfer. According to the currently accepted view of translation initiation, the first step is the binding of the initiator Met-tRNAi to the heterotrimeric GTP-binding initiation factor eIF2 to form the Met-tRNAi•eIF2•GTP ternary complex. The ternary complex then binds to the 40S ribosomal subunit containing bound initiation factor eIF3, a process that requires two other initiation factors eIF1 and eIF1A. This interaction leads to the formation of a 43S preinitiation complex (40S•eIF3•eIF1•eIF1A•Met-tRNAi•eIF2•GTP) which is then recruited to the 5′-capped end of the mRNA via...
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Algire MA, Maag D, Lorsch JR. Pi release from eIF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation. Mol Cell. 2005;20:251–62.
Asano K, Clayton J, Shalev J, Hinnebusch AG. A multifactor complex of eukaryotic initiation factors, elF1, elF2, elF3, elF5, and the initiator tRNA (Met) is an important translation intermediate in vivo. Genes Dev. 2000;14:2534–46.
Asano K, Krishnamoorthy T, Phan L, Pavitt GD, Hinnebusch AG. Conserved bipartite motifs in yeast eIF5 and eIF2B epsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2. EMBO J. 1999;18:1673–88.
Conte MR, Kelly G, Babon J, Sanfelice D, Youell J, Smerdon SJ, Proud CG. Structure of the eukaryotic initiation factor eIF5 reveals a fold common to several translation factors. Biochemistry. 2006;45:4550–8.
Das S, Maitra U. Mutational analysis of mammalian translation initiation factor 5 (eIF5): role of interaction between the beta subunit of eIF2 and eIF5 in eIF5 function in vitro and in vivo. Mol Cell Biol. 2000;20:3941–50.
Das S, Maitra U. Functional significance and mechanism of eIF5-promoted GTP hydrolysis in eukaryotic translation initiation. Prog Nucleic Acid Res Mol Biol. 2001;70:207–31.
Das S, Maiti T, Das K, Maitra U. Specific interaction of eukaryotic translation initiation factor 5 (eIF5) with the beta-subunit of eIF2. J Biol Chem. 1997;272:31712–8.
Das S, Ghosh R, Maitra U. Eukaryotic translation initiation factor 5 functions as a GTPase-activating protein. J Biol Chem. 2001;276:6720–6.
Donahue TF. Genetic approaches to translation initiation in Saccharomyces cerevisiae. In: Soneberg N, JWB H, Matthews MB, editors. Translational control of gene expression. New York: Cold Spring Harbor Press; 2000. p. 487–502.
Hinnebusch AG, Dever TE, Asano K. Mechanism of translation initiation in the yeast Saccharomyces cerevisiae. In: Mathews M, Sonneberg N, Hershey JWB, editors. Translational control in biology and medicine. New York: Cold Spring Harbor Laboratory Press; 2007. p. 225–68.
Huang HK, Yoon H, Hannig EM, Donahue TF. GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae. Genes Dev. 1997;11:2396–413.
Lorsch JR, Dever TE. Molecular view of 43S complex formation and start site selection in eukaryotic translation initiation. J Biol Chem. 2010;285:21203–7.
Maitra U, Stringer EA, Chaudhuri A. Initiation factors in protein biosynthesis. Annu Rev Biochem. 1982;51:869–900.
Majumdar R, Maitra U. Regulation of GTP hydrolysis prior to ribosomal AUG selection during eukaryotic translation initiation. EMBO J. 2005;24:3737–46.
Nanda JS, Cheung YN, Takacs JE, Martin-Marcos P, Saini AK, Hinnebusch AG, Lorsch JR. eIF1 controls multiple steps in start codon recognition during eukaryotic translation initiation. J Mol Biol. 2009;394:268–85.
Paulin FE, Campbell LE, O’Brien K, Loughlin J, Proud CG. Eukaryotic translation initiation factor 5 (eIF5) acts as a classical GTPase-activator protein. Curr Biol. 2001;11:55–9.
Pestova TV, Lomakin IB, Lee JH, Choi SK, Dever TE, Helen CUT. The joining of ribosomal subunits in eukaryotes requires eIF5B. Nature. 2000;403:332–5.
Pestova TV, Lorsch JR, Helen CUT. The mechanism of translation initiation in eukaryotes. In: Mathews M, Sonenberg N, Hershey JWB, editors. Translational control in biology and medicine. New York: Cold Spring Harbor Laboratory Press; 2007. p. 87–128.
Raychaudhuri P, Maitra U. Identification of ribosome-bound eukaryotic initiation factor 2•GDP binary complex as an intermediate in polypeptide chain initiation reaction. J Biol Chem. 1986;261:7723–8.
Sonenberg N, Hinnebusch AG. Regulation of translation initiation in eukaryotes: mechanisms and biological targets. Cell. 2009;136:731–45.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG
About this entry
Cite this entry
Maitra, U., Majumdar, R. (2018). TIF5 (eIF5). In: Choi, S. (eds) Encyclopedia of Signaling Molecules. Springer, Cham. https://doi.org/10.1007/978-3-319-67199-4_614
Download citation
DOI: https://doi.org/10.1007/978-3-319-67199-4_614
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-67198-7
Online ISBN: 978-3-319-67199-4
eBook Packages: Biomedical and Life SciencesReference Module Biomedical and Life Sciences