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Methanogens: Syntrophic Metabolism

  • Jessica R. Sieber
  • Michael J. McInerney
  • Nicolai Müller
  • Bernhard Schink
  • Rob P. Gunsalus
  • Caroline M. Plugge
Living reference work entry
Part of the Handbook of Hydrocarbon and Lipid Microbiology book series (HHLM)

Abstract

Syntrophy is a mutualistic interaction in which two metabolically different types of microorganisms are linked by the need to keep metabolites exchanged between the two partners at low concentrations to make the overall metabolism of both organisms feasible. In most cases, the cooperation is based on the transfer of hydrogen, formate, or acetate from fermentative bacteria to methanogens to make the degradation of electron-rich substrates thermodynamically favorable. Syntrophic metabolism proceeds at very low Gibbs’ free energy changes, close to the minimum free energy change needed to conserve energy biologically, which is the energy needed to transport one proton across the cytoplasmic membrane. Pathways for syntrophic degradation of fatty acids predict the net synthesis of about one-third of an ATP per round of catabolism. Syntrophic metabolism entails critical oxidation-reduction reactions in which H2 or formate production would be thermodynamically unfavorable unless energy is invested. Molecular insights into the membrane processes involved in ion translocation and reverse electron transport revealed that syntrophs harbor multiple systems for reverse electron transfer. While much evidence supports the interspecies transfer of H2 and formate, other mechanisms of interspecies electron transfer exist including cysteine cycling and possibly direct interspecies electron transfer as electric current via conductive pili or (semi)conductive minerals.

Notes

Acknowledgments

The work on syntrophic benzoate metabolism was supported by contract DE-FG02-96ER20214 from Physical Biosciences Division, Office of Science, US Department of Energy, and the work on reverse electron transfer was supported by National Science Foundation grant 1515843 to M.J.M. B. S., and N. M. are indebted to the German Research Foundation (DFG) for funding work on syntrophic butyrate oxidation and interspecies electron transfer.

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Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  • Jessica R. Sieber
    • 1
  • Michael J. McInerney
    • 2
  • Nicolai Müller
    • 3
  • Bernhard Schink
    • 3
  • Rob P. Gunsalus
    • 4
  • Caroline M. Plugge
    • 5
  1. 1.Department of BiologyUniversity of Minnesota-DuluthDuluthUSA
  2. 2.Department of Botany and MicrobiologyUniversity of OklahomaNormanUSA
  3. 3.Department of BiologyUniversität KonstanzKonstanzGermany
  4. 4.Department of Microbiology and Molecular GeneticsUCLALos AngelesUSA
  5. 5.Laboratory of MicrobiologyWageningen University & ResearchWageningenThe Netherlands

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