Evolving Enzymes for Biocatalysis

  • Uwe T. Bornscheuer
  • Robert Kourist
Reference work entry
Part of the Handbook of Hydrocarbon and Lipid Microbiology book series (HHLM)


This chapter covers concepts developed for the directed evolution of enzymes. The principle strategy is given in comparison to rational protein design followed by a description of the most prominent methods for creation of mutant libraries. Screening and selection strategies to identify the best hits in these libraries are presented followed by several assays developed for a range of enzyme classes. Finally, selected examples for the successful application of evolutionary methods to optimize biocatalysts are given.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Biotechnology and Enzyme Catalysis, Institute of BiochemistryGreifswald UniversityGreifswaldGermany
  2. 2.Institute of Molecular BiotechnologyGraz University of TechnologyGrazAustria

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