Screening for Enantioselective Enzymes

  • B. Franken
  • Karl-Erich Jaeger
  • J. Pietruszka
Reference work entry
Part of the Handbook of Hydrocarbon and Lipid Microbiology book series (HHLM)


A wealth of novel enzyme genes is accessible from natural sources, from genome and metagenome sequencing projects, and from libraries created by directed evolution. However, the identification of novel enzyme activities with a high potential for biotechnological applications still represents a major bottleneck. Here, assays are needed allowing to screen for enzyme activities and enantioselectivities. Hence, we describe several assays carried out on agar plates or in solution which are based on photometry, fluorometry, chromatography, electrophoresis, spectrometry, and spectroscopy.


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© Springer International Publishing AG, part of Springer Nature 2017

Authors and Affiliations

  1. 1.System SolutionsQiagen GmbHHildenGermany
  2. 2.Institute of Molecular Enzyme TechnologyHeinrich-Heine University Duesseldorf, Research Center JuelichJuelichGermany
  3. 3.Institute of Bioorganic ChemistryHeinrich-Heine University Duesseldorf, Research Center JuelichJuelichGermany

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