Natural Attenuation of Hydrocarbon Compounds in Groundwater
Natural attenuation (NA) is a widely used strategy for the risk-based management of groundwater contaminated with hydrocarbons. New process-based conceptual models highlight the spatial distribution of biodegradation processes in plumes, controlled by microbial activity, electron acceptor bioavailability, and aquifer properties, as a key control on NA performance. The plume fringe is identified as a critical interface for enhanced microbial activity and biodegradation of hydrocarbons in plumes. Mass transport and process heterogeneity in aquifers must be adequately resolved to ensure reliable estimates of hydrocarbon attenuation. The adoption of mass discharge as a performance measure and use of high-resolution multilevel monitoring devices is recommended to address these issues. These concepts are illustrated with a case study example. Stable isotope methods are important tools in NA assessments and, when combined with hydrochemical analyses, offer new possibilities to characterize contaminant sources, biodegradation pathways, redox processes, element cycling, the relative contribution of different attenuation processes, and quantify biodegradation of hydrocarbons in groundwater.
Natural attenuation (NA) of polluted subsurface environments refers to naturally occurring physical, chemical, and biological processes which occur in situ and act, in isolation or combination, without human intervention to reduce the mass, toxicity, mobility, flux, volume, or concentration of contaminants in soil and groundwater (Wiedemeier et al. 1995; ASTM 1998; U.S. EPA 1998a, b; Environment Agency 2000; McLaughlan et al. 2006). These in situ processes include biodegradation, abiotic degradation, dispersion, dilution, sorption, volatilization, and chemical or biological stabilization, transformation, or destruction (U.S. EPA 1999; Wiedemeier et al. 1999; McLaughlan et al. 2006; Rivett and Thornton 2008). The technical application of NA processes for the management of soil and contaminated groundwater is termed monitored natural attenuation (MNA). This emphasizes rigorous performance assessment of NA using appropriate monitoring strategies and analytical tools to demonstrate an acceptable reduction in environmental risk at a specific compliance point or receptor within a reasonable timeframe (U.S. EPA 1998a; Environment Agency 2000; Rügner et al. 2006). Example receptors include humans (by direct exposure), potable water supply wells, and surface water bodies, whereas the legal boundary of a site is a typical compliance point, although many other examples may be identified in specific circumstances. Different receptors may be identified at various stages in the management of a contaminated site, during future site development or use and for different contaminants, according to their properties. While NA can be implemented to manage inorganic contaminants in both soil and groundwater, this chapter describes the theoretical concepts and practical application for hydrocarbon compounds in groundwater. These are illustrated by reference to petroleum hydrocarbons, a common pollutant in groundwater, but also underpin the implementation of NA for other similar organic compounds (e.g., polyaromatic hydrocarbons, diesel, phenols, coal tar compounds). The reader is referred to other texts for the assessment of NA in soils (Mulligan and Yong 2004; Khan et al. 2011), for chlorinated organic compounds (RTDF 1997; U.S. EPA 1998b; Environment Agency 1999; Wiedemeier et al. 1999) and for inorganic compounds (U.S. EPA 2007a, b).
Scientifically and operationally NA is distinct from bioremediation in that only biological processes are considered in bioremediation, whereas these contribute with many other nonbiological processes to NA (Alvarez and Illman 2006; U.S. EPA 2006; Rivett and Thornton 2008). The key technical components underpinning the implementation of NA for groundwater are (i) a site-specific risk assessment, (ii) development of a conceptual model which describes NA processes and translation of this understanding into a mathematical model for prediction, and (iii) demonstration and performance assessment of NA against stated site management and remediation objectives (Wiedemeier et al. 1999; Environment Agency 2000; Alvarez and Illman 2006; McLaughlan et al. 2006). The risk assessment seeks to identify the relevant contaminant linkage(s) in terms of the source-pathway-receptor (or target) framework (Environment Agency 2006). Characterizing the source (e.g., type, form, distribution, quantity of contaminants) and quantifying NA processes along the pathway (e.g., aquifer) are critical to the robust prediction of potential impacts on receptors, risk reduction, and effectiveness of NA in groundwater. If this analysis indicates remediation is necessary at a site, then the identified risks can be mitigated to an acceptable level by reducing the source mass or concentration, and/or reducing the flux of contaminants or preventing migration along the pathway, and/or removing or isolating the receptor. In most cases source reduction measures will be required to support the implementation of NA for contaminated groundwater, to ensure the achievement of remediation objectives in reasonable timescales (Environment Agency 2000; Chapelle et al. 2003).
2 Conceptual Model for Natural Attenuation of Hydrocarbons in Groundwater
Groundwater contamination by hydrocarbons originates from sources (e.g., leaking underground storage tanks, pipeline distribution systems, refining facilities, coal-gasification and chemical manufacturing plants, fuel transportation, and waste disposal practices) that release pure or dissolved phase hydrocarbon compounds to soil or the unsaturated zone. The dissolved phase compounds migrate to the water table in recharge water, which infiltrates the unsaturated zone. Pure liquids move under gravity to the water table, where they either form an immiscible phase in the capillary fringe if less dense (light nonaqueous phase liquid, LNAPL) than water (e.g., petroleum fuel) (CL:AIRE 2014), or migrate below the water table if more dense (e.g., coal tar) (Environment Agency 2003). The constituent compounds also dissolve into groundwater, limited thermodynamically by their effective solubility in the mixture (Thornton et al. 2013).
Contaminated groundwater is transported from the source at A, through the plume B, to the receptor at C. If the flux and concentration of pollutants measured across the control planes is reduced sufficiently to make the risks to the receptor acceptably low, then NA is considered to be successful. The control planes are virtual points of reference, used at different locations along the plume flowpath to estimate the change in contaminant concentration or flux over the distance between them. This measure is used in more sophisticated monitoring strategies to assess the performance of NA for site management (see section on performance assessment). Biodegradation of the organic contaminants by aerobic respiration, nitrate reduction, and sulfate reduction using dissolved electron acceptors (O2, NO3, SO4) will occur at the periphery or fringe of the plume, driven by mixing between the plume and background groundwater (Lerner et al. 2000; Thornton et al. 2001a; Bauer et al. 2008; Gutierrez-Neri et al. 2009; Eckert et al. 2015). Slower anaerobic biodegradation by manganese reduction, iron reduction, and methanogenesis will occur inside the plume core, using mineral Mn and Fe oxide fractions on the aquifer sediment and fermentation processes. Generally, microbial activity and biodegradation processes at the plume fringe are more important for attenuation than processes occurring in the plume core (Thornton et al. 2001b; McLaughlan et al. 2006; Tuxen et al. 2006; Bauer et al. 2008; Tischer et al. 2013; Eckert et al. 2015). The plume will increase in length until a steady state is reached, in which the contaminant flux from the source is balanced by the rate of attenuation from the combined fringe mixing-controlled and the slower internal reactions (Chapelle et al. 2003; Gutierrez-Neri et al. 2009). Source management measures are typically implemented with NA to both reduce the duration and steady-state length of the plume (McLaughlan et al. 2006). If the source is depleted (e.g., by natural weathering) or removed through source management measures (e.g., remediation), the plume may become detached from the source and migrate down hydraulic gradient as a separate entity (Wiedemeier et al. 1999).
3 Sorption of Hydrocarbon Compounds in Groundwater
where Rf is the retardation factor for the organic chemical, relative to the groundwater velocity [dimensionless], ρb is the bulk density of aquifer material (M/L3), and θ is the porosity of the aquifer material (fraction).
Relative mobility of common organic chemicals in groundwater
4-Methylphenol ( p-cresol)
4 Biodegradation of Hydrocarbon Compounds in Groundwater
Biodegradation of hydrocarbon compounds in groundwater occurs by aerobic and anaerobic pathways, coupled to the reduction of dissolved electron acceptors (oxygen, nitrate, sulfate, and carbon dioxide) and mineral oxidants (manganese and iron oxides) on the aquifer sediment (Chapelle 1993; Borden 1994). In most cases, biodegradation of these compounds occurs through primary metabolism, in which the microorganisms use the hydrocarbon compounds as carbon and energy sources (Wiedemeier et al. 1999), although co-metabolism is also important (Borden 1994; Alvarez and Illman 2006). The various pathways by which hydrocarbon compounds can be biodegraded in groundwater to organic intermediates or mineralized to carbon dioxide and the microorganisms responsible have been extensively studied. A detailed analysis of these is outside the scope of this chapter, but can be found in many excellent reviews (Smith 1991; Cerniglia 1992; Harayama and Timmis 1992; Haritash and Kaushik 2009). This section describes the biogeochemical processes which develop in hydrocarbon-contaminated groundwater as a basis to interpret and predict the natural attenuation of these compounds.
Gibbs free energy of reaction for electron donor and electron acceptor half-cell reactions for selected hydrocarbon compounds
Electron donor half-cell reactiona
ΔGro (kJ/mol e-)
Electron acceptor half-cell reactionb
ΔGro (kJ/mol e‐)
C6H6 + 12H2O > 6CO2 + 30H+ + 30e−
2e− + H+ + C6Cl6 > C6HCl5 + Cl−
C7H8 + 14H2O > 7CO2 + 36H+ + 36e−
2e− + H+ + C6H2Cl4 > C6H3Cl3 + Cl−
C8H10 + 16H2O > 8CO2 + 42H+ + 42e−
2e− + H+ + C6H3Cl3 > C6H4Cl2 + Cl−
C6H4(CH3)2 + 16H2O > 8CO2 + 42H+ + 42e−
Redox process d
C6H3(CH3)3 + 18H2O > 9CO2 + 48H+ +48e−
4e− + 4H+ + O2 > 2H2O
C6H6O + 11H2O > 6CO2 + 28H+ + 28e−
5e− + 6H+ + NO3 − > N2 + 3H2O
C10H8 + 20H2O > 10CO2 + 48H+ + 48e−
2e− + 4H+ + MnO2(s) > Mn2+ + 2H2O
C6H2Cl4 + 12H2O > 6CO2 + 26H+ + 4Cl− + 22e−
e− + 3H+ + FeOOH > Fe2+ + 2H2O
C6H3Cl3 + 12H2O > 6CO2 + 27H+ + 3Cl− + 24e−
8e− + 8H+ + SO4 2− > S2− + 4H2O
C6H4Cl2 + 12H2O > 6CO2 + 28H+ + 2Cl− + 26e-
8e− + 8H+ + CO2(g) > CH4(g) + 2H2O
C6H5Cl + 12H2O > 6CO2 + 29H+ + Cl− + 28e−
CH2O + H2O > CO2 + 4H+ + 4e−
where [ ] denotes concentration or mass in moles, (aq) denotes aqueous concentration, and (s) denotes solid mineral species. A key assumption of this analysis is that all electron acceptors are available for biodegradation. However, biodegradation using dissolved electron acceptors is potentially limited by the relatively low solubility of oxygen or rate of mass transfer of oxygen, nitrate, and sulfate into the plume by dispersion (Thornton et al. 2001a, b; 2014). Moreover, while aquifer sediments typically have a significant reservoir of mineral-bound electron acceptors as Mn- and Fe-oxides, only the easily reducible amorphous forms will contribute oxidation capacity for hydrocarbon biodegradation in groundwater (Borden 1994; Wiedemeier et al. 1999; Banwart and Thornton 2003; Thornton et al. 2011). Estimates of OXC therefore represent an upper limit on the maximum concentration of hydrocarbons that can be biodegraded according to the theoretical consumption of the total mass of electron acceptors in the aquifer.
5 Performance Assessment of Natural Attenuation for Hydrocarbon Compounds
Data collection and analysis typically used for the performance assessment of hydrocarbon natural attenuation in groundwater
Indicator or measurement
Basis for performance assessment
Decreased electron acceptor concentration
Utilization by microorganisms during biodegradation of specific hydrocarbons
Increased inorganic carbon concentration
Production from biodegradation and mineralization of hydrocarbon compounds and organic metabolites
Stoichiometry and mass balance between reactants and products
Balanced consumption of electron acceptors and organic compounds (hydrocarbons and relevant organic metabolites) during biodegradation under specific redox conditions
Increased concentrations of intermediate-stage and final products
Identification of specific biodegradation pathways and transformation for hydrocarbons
Increased ratio of transformation products to parent compounds
Progressive biodegradation of parent compound to metabolite over space and time
Decreased ratio of reactant to inert tracer
Preferential biodegradation of hydrocarbon species relative to a nonreactive species in the same mixture, accounting natural attenuation due to abiotic processes
Relative rates of transformation of different hydrocarbons consistent with laboratory data
Independent confirmation of biodegradation potential at field-scale based on controlled laboratory studies
Changes in carbon, chloride, and hydrogen isotope ratios of specific organic contaminants (depending on respective chemical group), and isotope ratios of electron acceptors and inorganic carbon in CO2 and CH4
Biodegradation of specific organic compounds (if based on compound-specific isotope analysis, CSIA), biodegradation of nonspecific compounds (if based on total dissolved carbon isotope ratios), and specific redox processes (if based on isotope ratios of electron acceptors)
Increased number of (live/active) bacteria in hydrocarbon plume
Increase in size of viable microbial community for in situ biodegradation
Increase in relative abundance of known degraders in hydrocarbon plume
Increased contribution of specific microorganisms within community with capability for hydrocarbon biodegradation
Increase in abundance of genes associated with biodegradation in hydrocarbon plume
Development of metabolic pathways in microorganisms which enable biodegradation of specific hydrocarbons
Primary line of evidence: Field time-series data showing a consistent reduction in the contaminant concentration or flux over time at one or more points along the source-pathway-receptor linkage, typically used to deduce the status of the plume (i.e., expanding, stable or shrinking), relative to predictions of groundwater flow
Secondary line of evidence: Field data demonstrating in situ biodegradation of hydrocarbons in the plume, typically based on the consumption of dissolved (e.g., O2, NO3, SO4) and mineral phase (e.g., MnO4, FeOOH) electron acceptors in the aquifer and corresponding increase in organic metabolites, inorganic reaction products (e.g., Mn2+, Fe2+, HS−), and other chemical species (e.g., dissolved CO2 and CH4) that verify different biodegradation processes
Tertiary line of evidence: Supporting evidence related to the verification of natural attenuation processes, such as laboratory microcosm studies to quantify biodegradation rates, confirmation of biodegradation mechanisms and pathways using stable isotope analysis, or the use of molecular biological techniques to demonstrate the activity of appropriate hydrocarbon-degrading microorganisms
Not all lines of evidence are required in every case, but rather an appropriate level of data collection and interpretation will apply. However, this analysis will typically include mathematical modeling studies to verify the site conceptual model underpinning the interpretation and to predict the long-term behavior of the plume for site management. Primary and secondary lines of evidence are obtained from the distribution of dissolved reactants along the plume flowpath, using groundwater samples collected from monitoring wells in the plume source area, uncontaminated and contaminated sections of the aquifer. The plume is presumed to have a centerline, characterized by the monitoring wells, for this analysis. Visual, graphical, and quantitative methods, including regression techniques, statistical analyses, and mass balances (e.g., Table 3), are available to interpret these data using concentration versus time and concentration versus distance plots (see Wiedemeier et al. 1999; Environment Agency 2000; Alvarez and Illman 2006 for summary). An important objective is the estimation of plume-scale contaminant biodegradation rates from this analysis, often assumed to follow first-order kinetics for mathematical simplicity and use in numerical reactive transport codes (Buscheck and Alcantar 1995; Suarez and Rifai 1999; Beyer et al. 2007). Similarly, the maximum steady-state plume length and time to plume stabilization must also be predicted, usually to evaluate receptor impacts and remediation timescales. Various mathematical approaches and modeling tools have been developed to undertake this for hydrocarbons plumes, based on different conceptual models of plume development, substrate mixtures, and biodegradation processes (Chapelle et al. 2003; Atteia and Guillot 2007; Gutierrez-Neri et al. 2009).
A relatively sophisticated site assessment and monitoring program is required to verify the effectiveness of NA for hydrocarbons in groundwater (Wiedemeier et al. 2006). This is because the technical appraisal relies primarily on the evaluation of subsurface transport and biodegradation processes using the aquifer characteristics and groundwater quality data. However, data quality can be strongly influenced by heterogeneity in the spatial and temporal distribution of contaminants, geological and hydrogeological properties, and biodegradation processes, which must be resolved for correct performance assessment of NA. These issues are incorporated in the concepts of mass transport heterogeneity and process heterogeneity, which must be resolved in NA assessments.
5.1 Mass Transport Heterogeneity
Hydrocarbon releases from source zones may also vary temporally as the source term naturally weathers or is depleted in more soluble components (Thornton et al. 2013), or due to water table fluctuations, recharge variations, and changes in groundwater flow direction (McMahon and Bruce 1997; Davis et al. 1999; McGuire et al. 2000; Mackay et al. 2001; Wilson et al. 2002). This variability may occur on a scale of weeks to months (water table, recharge and flow direction changes) or years (source term aging and compositional changes). Therefore, centerline monitoring typically requires more detailed site characterization to accurately define the spatial-temporal variation in plume flowpath.
The effect of mass transport heterogeneity on NA assessments is potentially significant. The assumption of a fixed centerline for a plume can mean that the primary pathway contributing most to contaminant migration can be missed. When the monitoring wells which intersect the plume are sampled, this can result in an apparent longitudinal contaminant concentration profile, which is less than the actual profile (Fig. 3a–c). A misleading interpretation of a much shorter plume length and overestimation of hydrocarbon attenuation along the flowpath would then arise.
An alternative approach is to use changes in contaminant mass discharge and mass flux through a series of monitoring wells set transverse to the plume flowpath (i.e., functioning as control planes) and capturing the full depth and width of the plume for performance assessment of NA (Kao and Wang 2001; Wilson et al. 2004; Alvarez and Illman 2006; ITRC 2010). Mass discharge and flux estimates quantify source or plume strength at a given time and location (ITRC 2010). Mass flux is a rate measurement specific to a defined area, which is usually a subset of a plume cross section, and expressed as mass/time/area. Mass discharge is an integrated mass flux estimate, representing the sum of all mass flux measures across an entire plume. It therefore gives the total mass of any solute transported in groundwater through a defined plane. If the total mass discharge of contaminants migrating through successive control planes decreases over time, NA is evident (Alvarez and Illman 2006).
where K is the hydraulic conductivity of the flow area (L/T) and i is the aquifer hydraulic gradient (L/L).
Flux planes can capture spatially and temporally variable contaminant distribution, as well as estimate biodegradation rates using different methods, such as the sampling of a dense monitoring array (Borden et al. 1997; Kao and Wang 2001; Béland-Pelletier et al. 2011), control plane pumping of a more sparse array (Bockelmann et al. 2003; ITRC 2010; Béland-Pelletier et al. 2011), or a passive flux meter approach (Hatfield et al. 2004; ITRC 2010). In addition to defining the source strength and plume attenuation rate, mass flux estimates can identify areas of a plane through which most contaminant mass is moving. This helps target monitoring and remediation efforts (e.g., amendments to enhance NA) to optimize treatment. As such, flux-based methods provide a superior analysis of NA performance compared with methods based on assumed centerlines of plumes, by integrating results from many monitoring wells and accommodating plume heterogeneity in estimates of mass loss (API 2003; Wilson et al. 2004; Alvarez and Illman 2006; Beyer et al. 2007). However, this approach requires a higher density of groundwater monitoring wells or sampling stations, ideally using higher resolution MLS, to characterize the contaminant and hydraulic conductivity distribution in sufficient detail (API 2003; Alvarez and Illman 2006; ITRC 2010).
5.2 Process Heterogeneity
Process heterogeneity refers to the spatial and temporal distribution of biodegradation processes that occurs in plumes. As biodegradation is the most important attenuation mechanism for hydrocarbon compounds in groundwater, monitoring should focus on deducing the type and location of biodegradation processes in plumes from the groundwater chemistry and other data. This information is necessary to estimate biodegradation rates (Thornton et al. 2001b), and experience shows that contaminant biodegradation is strongly influenced by the evolution in redox conditions (Lyngkilde and Christensen 1992; Lerner et al. 2000; Cozzarelli et al. 2001; Thornton et al. 2001a), contaminant concentrations (Pickup et al. 2001; Wu et al. 2006; Baker et al. 2012) and microbial communities (Elliott et al. 2010; Rizoulis et al. 2013) in plumes. While the characteristic longitudinal development of redox zones resulting from biodegradation of hydrocarbon compounds in groundwater (Fig. 2) has long been known (Champs et al. 1979; Barcelona et al. 1989; Chapelle et al. 2002), the importance of biodegradation at the plume fringe is now increasingly recognized as a major contribution to overall biodegradation in organic contaminant plumes (Pickup et al. 2001; Anneser et al. 2008; Prommer et al. 2009; Tischer et al. 2013; Thornton et al. 2014). However, weak vertical dispersive mixing means that the plume fringe zone may be less than 1 m thick (Davis et al. 1999; Schreiber and Bahr 1999; Cozzarelli et al. 1999; Cozzarelli et al. 2001; McGuire et al. 2000), with steep gradients in chemical species, such as electron acceptors, electron donors, and metabolites (see profiles in Fig. 2), and microorganisms supporting biodegradation (Tuxen et al. 2006).
Contaminant carbon and aquifer oxidant consumption estimated from MLS vertical profiles at locations A and B
Oxidant consumption (kg·m−2)a
Contaminant consumption (kg phenol-C·m−2)a, b
5.3 Stable Isotopes
Measurements of hydrochemical species in groundwater will not always provide conclusive proof of hydrocarbon biodegradation for NA assessments (Borden 1994; Hunkeler and Aravena 2010). For example, biodegradation of specific hydrocarbon compounds in mixtures can be difficult to deduce if common pathways exist, when organic metabolites are similar to potential parent compounds, or if analytes are present at trace levels that are difficult to quantify (Alvarez and Illman 2006; Spence et al. 2005). Also changes in redox conditions which are attributed to hydrocarbon biodegradation in groundwater can also arise from metabolism of other co-contaminants, including natural substances, confounding quantitative interpretation, and mass balances at the plume scale.
Many of these problems can be addressed using natural abundance and compound-specific stable isotope analysis (CSIA) of H, C, N, O, S, and Cl in hydrocarbon compounds, their biodegradation metabolites, and the respective elements in NO3 −, SO4 2−, CO2, CH4. The fundamental principles and environmental applications of stable isotope analysis have been extensively reviewed (Meckenstock et al. 2004; Hunkeler and Elsner 2010; Elsner and Imfeld 2016). With respect to the natural attenuation of hydrocarbons in groundwater, stable isotope analysis can be used to (i) identify the source(s) and origin (e.g., via biological or abiotic transformation) of a compound (both organic and inorganic) released to or formed in a given environment; (ii) deduce specific pathways and redox processes (e.g., aerobic vs. anaerobic) for biodegradation; (iii) assess the contribution of biotic and abiotic transformation processes affecting contaminants during subsurface transport; (iv) quantify the extent of biodegradation; (v) verify reactive transport models for prediction of environmental impacts, remediation system design, and treatment timescales; and (vi) understand element cycling in natural and contaminated systems using isotope-labeled compounds (Höhener and Aelion 2010).
More recent development of stable isotope methods include the use of dual isotope analysis to interpret in situ biodegradation processes for aromatic hydrocarbons and other organic contaminants. Attention has mainly focused on understanding the reaction mechanisms and enzymes involved (Morasch et al. 2001), relevant metabolic pathways (Mancini et al. 2003; Fischer et al. 2008), characterizing the physiological groups of microorganisms facilitating biodegradation (Kümmel et al. 2015), deducing the dominant redox processes supporting biodegradation (Spence et al. 2001, 2005; Feisthauer et al. 2012; Thornton et al. 2014), and quantifying biodegradation at field scale (Hunkeler et al. 2001; Mancini et al. 2002, 2003; Richnow et al. 2003; Griebler et al. 2004; Meckenstock et al. 2004; Spence et al. 2005; Fischer et al. 2007; Hunkeler and Aravena 2010; Thornton et al. 2011; Lesser-Carillo 2014).
6 Research Needs
The process-based conceptual model which describes the spatial distribution of microbiological activity and biodegradation potential in hydrocarbon plumes is increasingly recognized as a fundamental biogeochemical framework to understand and predict the natural attenuation of a wide range of aromatic hydrocarbon contaminants in groundwater. The steep gradients in biogeochemical processes which characterize the plume fringe interface provide a unique environment for the biodegradation of oxidizable organic chemicals. However, further research is needed in two areas to understand the complex relationships that exist within the microbial communities which develop at this interface and to interpret their interactions in response to changing environmental conditions.
6.1 Mechanistic Understanding of Microbial Community Interactions at Plume Interfaces
It is likely that the composition, function, and activity of specific populations within the microbial community at the plume fringe will be sensitive to selective pressures created by the availability of organic substrates and electron acceptors, which in turn are controlled by the aquifer properties and groundwater flow field. Both competitive and synergistic interactions between microbial populations may characterize the biodegradation of hydrocarbons under these conditions, but this is poorly understood. Moreover, little is known about the characteristic response times of the microbial community to temporal variations in plume conditions, such as those which occur by natural evolution in the source term composition and by engineered interventions (e.g., injection of amendments to increase microbial activity and contaminant turnover). The work of Thornton et al. (2014) highlighted the potential to increase rates of NA in such circumstances, but the response, in particular the recovery timescales, of planktonic and attached populations in a hydrocarbon plume to such intervention must be qualified and formerly linked to the biogeochemical drivers controlling this behavior.
6.2 Quantitative Description of Microbial Interactions at Plume Interfaces
Datasets to quantitatively measure and interpret the microbial interactions which develop at the plume fringe, supported by a theoretical framework which enables these observations to be translated into predictions of NA performance at field scale. This necessarily requires the improved mechanistic understanding outlined above and new modeling tools which can represent such dynamic relationships across different scales (microscale to plume scale). The work completed in Watson et al. (2005) highlighted the importance of such adaptive modeling frameworks to scale biogeochemical processes across the plume fringe but also emphasized the conceptual and theoretical limitations of existing modeling approaches.
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