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Intratesticular Sperm Extraction

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Endocrinology of the Testis and Male Reproduction

Part of the book series: Endocrinology ((ENDOCR))

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Abstract

In the scenario of medically assisted reproduction, sperm retrieval techniques (SRTs) represent a concrete possibility to find, freeze, and use spermatozoa in men with a diagnosis of azoospermia. Therefore, many infertile couples because of a severe male factor can achieve a biological offspring.

Nowadays the surgical overview in this field has a number of available techniques according to the causal and baseline condition. These stretch from the percutaneous approach to the latest and more sophisticated microsurgical dissection techniques aimed at increasing surgical specificity and eventually the sperm retrieval rate in individuals with nonobstructive azoospermia. In this context, the careful and complete diagnostic classification of every patient (obstructive vs. nonobstructive azoospermia) is essential in the choice of the surgical approach (percutaneous vs. open) and the anatomical site of acquisition (epididymis vs. testis), as well as in the prediction of the sperm retrieval successful rate.

Although can be considered day-surgery procedures, SRTs are clearly not free from potential sequelae and complications; therefore, it is clinically relevant to have an appropriate surgical indication, a thorough information of the patient and a meticulous perioperative management.

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References

  • Abdel Raheem A, Garaffa G, Rushwan N, De Luca F, Zacharakis E, Abdel Raheem T, Freeman A, Serhal P, Harper JC, Ralph D. Testicular histopathology as a predictor of a positive sperm retrieval in men with non-obstructive azoospermia. BJU Int. 2013;111:492–9.

    Article  PubMed  Google Scholar 

  • Ali Jorsaraei SG, Shafi H, Alereza H, Esmailzadeh S, Delavar MA, Saee A, Fasihiyan M, Golsorkhtabaramiri M. Azoospermia and testicular biopsy before intra-cytoplasmic sperm injection: does the type of anesthesia make a difference? J Nat Sci Biol Med. 2016;7:89–92.

    Article  PubMed  PubMed Central  Google Scholar 

  • Amer M, Ateyah A, Hany R, Zohdy W. Prospective comparative study between microsurgical and conventional testicular sperm extraction in non-obstructive azoospermia: follow-up by serial ultrasound examinations. Hum Reprod. 2000;15:653–6.

    Article  CAS  PubMed  Google Scholar 

  • Bernie AM, Mata DA, Ramasamy R, Schlegel PN. Comparison of microdissection testicular sperm extraction, conventional testicular sperm extraction, and testicular sperm aspiration for nonobstructive azoospermia: a systematic review and meta-analysis. Fertil Steril. 2015;104:1099–103.e1-3.

    Google Scholar 

  • Bromage SJ, Falconer DA, Lieberman BA, Sangar V, Payne SR. Sperm retrieval rates in subgroups of primary azoospermic males. Eur Urol. 2007;51(2):534–9.

    Article  PubMed  Google Scholar 

  • Caroppo E, Colpi EM, Gazzano G, Vaccalluzzo L, Scroppo FI, D’Amato G, Colpi GM. Testicular histology may predict the successful sperm retrieval in patients with non-obstructive azoospermia undergoing conventional TESE: a diagnostic accuracy study. J Assist Reprod Genet. 2017;34:149–54.

    Article  PubMed  Google Scholar 

  • Carpi A, Fabris GF, Chiechi A, Nardini V, Romani R, Di Coscio G. Spermatogenesis in azoospermic, formerly cryptorchid men. Use of needle aspiration techniques. Acta Cytol. 2002;46:848–54.

    Article  PubMed  Google Scholar 

  • Carpi A, Sabanegh E, Mechanick J. Controversies in the management of nonobstructive azoospermia. Fertil Steril. 2009;91:963–70.

    Article  PubMed  Google Scholar 

  • Cissen M, Meijerink AM, D’Hauwers KW, Meissner A, van der Weide N, Mochtar MH, de Melker AA, Ramos L, Repping S, Braat DD, Fleischer K, van Wely M. Prediction model for obtaining spermatozoa with testicular sperm extraction in men with non-obstructive azoospermia. Hum Reprod. 2016;31:1934–41.

    Article  CAS  PubMed  Google Scholar 

  • Craft I, Shrivastav P. Treatment of male infertility. Lancet. 1994;344(8916):191–2.

    Article  CAS  PubMed  Google Scholar 

  • Deruyver Y, Vanderschueren D, Van der Aa F. Outcome of microdissection TESE compared with conventional TESE in non-obstructive azoospermia: a systematic review. Andrology. 2014;2:20–4.

    Article  CAS  PubMed  Google Scholar 

  • Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, Van Steirteghem A, Silber S. Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia. Hum Reprod. 1995;10:1457–60.

    Article  CAS  PubMed  Google Scholar 

  • Donoso P, Tournaye H, Devroey P. Which is the best sperm retrieval technique for non-obstructive azoospermia? A systematic review. Hum Reprod Update. 2007;13:539–49.

    Article  CAS  PubMed  Google Scholar 

  • EAU Guidelines on Male Infertility. 2016. https://uroweb.org/wp-content/uploads/EAU-Guidelines-Male-Infertility-2016.pdf

  • Esteves SC. Percutaneous epididymal sperm aspiration as a method for sperm retrieval in men with obstructive azoospermia seeking fertility: operative and laboratory aspects. Int Braz J Urol. 2015;41:817.

    Article  PubMed  PubMed Central  Google Scholar 

  • Esteves SC, Miyaoka R, Agarwal A. Sperm retrieval techniques for assisted reproduction. Int Braz J Urol. 2011;37:570–83.

    Article  PubMed  Google Scholar 

  • Esteves SC, Miyaoka R, Orosz JE, Agarwal A. An update on sperm retrieval techniques for azoospermic males. Clinics. 2013a;68(Suppl 1):99–110.

    Article  PubMed  PubMed Central  Google Scholar 

  • Esteves SC, Lee W, Benjamin DJ, Seol B, Verza Jr S, Agarwal A. Reproductive potential of men with obstructive azoospermia undergoing percutaneous sperm retrieval and intracytoplasmic sperm injection according to the cause of obstruction. J Urol. 2013b;189:232–7.

    Article  PubMed  Google Scholar 

  • Ezeh UI, Taub NA, Moore HD, Cooke ID. Establishment of predictive variables associated with testicular sperm retrieval in men with non-obstructive azoospermia. Hum Reprod. 1999;14:1005–12.

    Article  CAS  PubMed  Google Scholar 

  • Fasouliotis SJ, Safran A, Porat-Katz A, Simon A, Laufer N, Lewin A. A high predictive value of the first testicular fine needle aspiration in patients with non-obstructive azoospermia for sperm recovery at the subsequent attempt. Hum Reprod. 2002;17:139–42.

    Article  CAS  PubMed  Google Scholar 

  • Franco G, Scarselli F, Casciani V, De Nunzio C, Dente D, Leonardo C, Greco PF, Greco A, Minasi MG, Greco E. A novel stepwise micro-TESE approach in non obstructive azoospermia. BMC Urol. 2016;16:20.

    Article  PubMed  PubMed Central  Google Scholar 

  • Friedler S, Raziel A, Strassburger D, Soffer Y, Komarovsky D, Ron-El R. Testicular sperm retrieval by percutaneous fine needle sperm aspiration compared with testicular sperm extraction by open biopsy in men with non-obstructive azoospermia. Hum Reprod. 1997;12:1488–93.

    Article  CAS  PubMed  Google Scholar 

  • Ghalayini IF, Al-Ghazo MA, Hani OB, Al-Azab R, Bani-Hani I, Zayed F, Haddad Y. Clinical comparison of conventional testicular sperm extraction and microdissection techniques for non-obstructive azoospermia. J Clin Med Res. 2011;3:124–31.

    PubMed  PubMed Central  Google Scholar 

  • Gies I, Oates R, De Schepper J, Tournaye H. Testicular biopsy and cryopreservation for fertility preservation of prepubertal boys with Klinefelter syndrome: a pro/con debate. Fertil Steril. 2016;105:249–55.

    Article  PubMed  Google Scholar 

  • Gorgy A, Meniru GI, Naumann N, Beski S, Bates S, Craft IL. The efficacy of local anaesthesia for percutaneous epididymal sperm aspiration and testicular sperm aspiration. Hum Reprod. 1998;13:646–50.

    Article  CAS  PubMed  Google Scholar 

  • Harrington TG, Schauer D, Gilbert BR. Percutaneous testis biopsy: an alternative to open testicular biopsy in the evaluation of the subfertile man. J Urol. 1996;156:1647–51.

    Article  CAS  PubMed  Google Scholar 

  • Janzen N, Goldstein M, Schlegel PN, Palermo GD, Rosenwaks Z, Hariprashad J. Use of electively cryopreserved microsurgically aspirated epididymal sperm with IVF and intracytoplasmic sperm injection for obstructive azoospermia. Fertil Steril. 2000;74:696–701.

    Article  CAS  PubMed  Google Scholar 

  • Jensen CF, Ohl DA, Hiner MR, Fode M, Shah T, Smith GD, Sonksen J. Multiple needle-pass percutaneous testicular sperm aspiration as first-line treatment in azoospermic men. Andrology. 2016;4:257–62.

    Article  CAS  PubMed  Google Scholar 

  • Khadra AA, Abdulhadi I, Ghunain S, Kilani Z. Efficiency of percutaneous testicular sperm aspiration as a mode of sperm collection for intracytoplasmic sperm injection in nonobstructive azoospermia. J Urol. 2003;169:603–5.

    Article  PubMed  Google Scholar 

  • Lewin A, Weiss DB, Friedler S, Ben-Shachar I, Porat-Katz A, Meirow D, Schenker JG, Safran A. Delivery following intracytoplasmic injection of mature sperm cells recovered by testicular fine needle aspiration in a case of hypergonadotropic azoospermia due to maturation arrest. Hum Reprod. 1996;11:769–71.

    Article  CAS  PubMed  Google Scholar 

  • Lewin A, Reubinoff B, Porat-Katz A, Weiss D, Eisenberg V, Arbel R, Bar-el H, Safran A. Testicular fine needle aspiration: the alternative method for sperm retrieval in non-obstructive azoospermia. Hum Reprod. 1999;14:1785–90.

    Article  CAS  PubMed  Google Scholar 

  • Martin-du-Pan RC, Bischof P. Increased follicle stimulating hormone in infertile men. Is increased plasma FSH always due to damaged germinal epithelium? Hum Reprod. 1995;10:1940–5.

    Article  CAS  PubMed  Google Scholar 

  • Mascarenhas M, Thomas S, Kamath MS, Ramalingam R, Kongari AM, Yuvarani S, Srivastava VM, George K. Prevalence of chromosomal abnormalities and Y chromosome microdeletion among men with severe semen abnormalities and its correlation with successful sperm retrieval. J Hum Reprod Sci. 2016;9:187–93.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Miyamoto T, Minase G, Okabe K, Ueda H, Sengoku K. Male infertility and its genetic causes. J Obstet Gynaecol Res. 2015;41:1501–5.

    Article  CAS  PubMed  Google Scholar 

  • Nudell DM, Conaghan J, Pedersen RA, Givens CR, Schriock ED, Turek PJ. The mini-micro-epididymal sperm aspiration for sperm retrieval: a study of urological outcomes. Hum Reprod. 1998;13:1260–5.

    Article  CAS  PubMed  Google Scholar 

  • Okada H, Dobashi M, Yamazaki T, Hara I, Fujisawa M, Arakawa S, Kamidono S. Conventional versus microdissection testicular sperm extraction for nonobstructive azoospermia. J Urol. 2002;168:1063–7.

    Article  PubMed  Google Scholar 

  • Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340(8810):17–8.

    Article  CAS  PubMed  Google Scholar 

  • Plotton I, Giscard d’Estaing S, Cuzin B, Brosse A, Benchaib M, Lornage J, Ecochard R, Dijoud F, Lejeune H, FERTIPRESERVE group. Preliminary results of a prospective study of testicular sperm extraction in young versus adult patients with nonmosaic 47,XXY Klinefelter syndrome. J Clin Endocrinol Metab. 2015;100(3):961–7.

    Article  CAS  PubMed  Google Scholar 

  • Ramasamy R, Yagan N, Schlegel PN. Structural and functional changes to the testis after conventional versus microdissection testicular sperm extraction. Urology. 2005;65:1190–4.

    Article  PubMed  Google Scholar 

  • Ramasamy R, Lin K, Gosden LV, Rosenwaks Z, Palermo GD, Schlegel PN. High serum FSH levels in men with nonobstructive azoospermia does not affect success of microdissection testicular sperm extraction. Fertil Steril. 2009a;92:590–3.

    Article  CAS  PubMed  Google Scholar 

  • Ramasamy R, Ricci JA, Palermo GD, Gosden LV, Rosenwaks Z, Schlegel PN. Successful fertility treatment for Klinefelter’s syndrome. J Urol. 2009b;182:1108–13.

    Article  CAS  PubMed  Google Scholar 

  • Rohayem J, Fricke R, Czeloth K, Mallidis C, Wistuba J, Krallmann C, Zitzmann M, Kliesch S. Age and markers of Leydig cell function, but not of Sertoli cell function predict the success of sperm retrieval in adolescents and adults with Klinefelter’s syndrome. Andrology. 2015;3:868–75.

    Article  CAS  PubMed  Google Scholar 

  • Saccà A, Pastore AL, Roscigno M, Naspro R, Pellucchi F, Fuschi A, Maruccia S, Territo A, Pisano F, Zanga L, Capitanio E, Carbone A, Fusi F, Chinaglia D, Da Pozzo LF. Conventional testicular sperm extraction (TESE) and non-obstructive azoospermia: is there still a chance in the era of microdissection TESE? Results from a single non-academic community hospital. Andrology. 2016;4:425–9.

    Article  PubMed  Google Scholar 

  • Schiff JD, Palermo GD, Veeck LL, Goldstein M, Rosenwaks Z, Schlegel PN. Success of testicular sperm extraction [corrected] and intracytoplasmic sperm injection in men with Klinefelter syndrome. J Clin Endocrinol Metab. 2005;90:6263–7.

    Article  CAS  PubMed  Google Scholar 

  • Schlegel PN. Testicular sperm extraction: microdissection improves sperm yield with minimal tissue excision. Hum Reprod. 1999;14:131–5.

    Article  CAS  PubMed  Google Scholar 

  • Shin DH, Turek PJ. Sperm retrieval techniques. Nat Rev Urol. 2013;10:723–30.

    Article  PubMed  Google Scholar 

  • Silber SJ, Nagy ZP, Liu J, Godoy H, Devroey P, Van Steirteghem AC. Conventional in-vitro fertilization versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration. Hum Reprod. 1994;9:1705–9.

    Article  CAS  PubMed  Google Scholar 

  • Takeda T, Iwatsuki S, Hamakawa T, Mizuno K, Kamiya H, Umemoto Y, Kubota H, Kubota Y, Sasaki S, Yasui T. Chromosomal anomalies and sperm retrieval outcomes of patients with non-obstructive azoospermia: a case series. Andrology. 2017. doi:10.1111/andr.12338. [Epub ahead of print].

    PubMed  Google Scholar 

  • Temple-Smith PD, Southwick GJ, Yates CA, Trounson AO, de Kretser DM. Human pregnancy by in vitro fertilization (IVF) using sperm aspirated from the epididymis. J In Vitro Fert Embryo Transf. 1985;2:119–22.

    Article  CAS  PubMed  Google Scholar 

  • Tournaye H, Krausz C, Oates RD. Novel concepts in the aetiology of male reproductive impairment. Lancet Diabetes Endocrinol. 2016a. doi:10.1016/S2213-8587(16)30040-7. pii: S2213-8587(16)30040-7.

    Google Scholar 

  • Tournaye H, Krausz C, Oates RD. Concepts in diagnosis and therapy for male reproductive impairment. Lancet Diabetes Endocrinol. 2016b. doi:10.1016/S2213-8587(16)30043-2. pii: S2213-8587(16)30043-2.

    Google Scholar 

  • Tsujimura A, Matsumiya K, Miyagawa Y, Tohda A, Miura H, Nishimura K, Koga M, Takeyama M, Fujioka H, Okuyama A. Conventional multiple or microdissection testicular sperm extraction: a comparative study. Hum Reprod. 2002;17:2924–9.

    Article  CAS  PubMed  Google Scholar 

  • Tsujimura A, Matsumiya K, Miyagawa Y, Takao T, Fujita K, Koga M, Takeyama M, Fujioka H, Okuyama A. Prediction of successful outcome of microdissection testicular sperm extraction in men with idiopathic nonobstructive azoospermia. J Urol. 2004;172:1944–7.

    Article  PubMed  Google Scholar 

  • Turek PJ, Givens CR, Schriock ED, Meng MV, Pedersen RA, Conaghan J. Testis sperm extraction and intracytoplasmic sperm injection guided by prior fine-needle aspiration mapping in patients with nonobstructive azoospermia. Fertil Steril. 1999;71:552–7.

    Article  CAS  PubMed  Google Scholar 

  • Van Rumste MM, Evers JL, Farguhar CM. Intra-cytoplasmic sperm injection versus conventional techniques for oocyte insemination during in vitro fertilization in patients with non-male subfertility. Cochrane Database Syst Rev. 2003;2:CD001301.

    Google Scholar 

  • Wosnitzer M, Goldstein M, Hardy MP. Review of azoospermia. Spermatogenesis. 2014;4:e28218.

    Article  PubMed  PubMed Central  Google Scholar 

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Correspondence to Andrea Salonia .

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Oreggia, D., Ventimiglia, E., Salonia, A. (2017). Intratesticular Sperm Extraction. In: Simoni, M., Huhtaniemi, I. (eds) Endocrinology of the Testis and Male Reproduction. Endocrinology. Springer, Cham. https://doi.org/10.1007/978-3-319-44441-3_40

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  • DOI: https://doi.org/10.1007/978-3-319-44441-3_40

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