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Novel Approaches for Detection of Bacteriophage

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Bacteriophages

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Abstract

Modern analytical instrumentation combined with increased computational power has dramatically enhanced the capacity to interrogate the physicochemical properties of macromolecular structures. For example, it has become routine to analyze complex protein mixtures by mass spectrometry and quantitatively identify thousands of proteins in the nanomolar to picomolar range in a single analytical run. The relatively small size and simple structure of bacteriophages, generally consisting of a protein coat surrounding a nucleic acid core, make them highly amenable to examination by these new analytical methods, and assays can be developed that exploit inherent bacteriophage functionality. This chapter describes the recent utilization of bacteriophages in conjunction with mass spectrometry and Raman spectroscopy for direct detection of bacteriophages.

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Authors and Affiliations

  1. Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA, 30341, USA

    Carrie L. Pierce, Jon C. Rees & John R. Barr

Authors
  1. Carrie L. Pierce
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  2. Jon C. Rees
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  3. John R. Barr
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Corresponding author

Correspondence to Carrie L. Pierce .

Editor information

Editors and Affiliations

  1. Evolution Biotechnologies , Evolution Biotechnologies, United Kingdom

    David Harper

  2. Dept. of Microbiology, The Ohio State University, Mansfield, Ohio, USA

    Stephen Abedon

  3. GeneWEAVE Biosciences, Roche Molecular Systems GeneWEAVE Biosciences, Los Gatos, USA

    Benjamin Burrowes

  4. Evolution Biotechnologies , Edinburgh, United Kingdom

    Malcolm McConville

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© 2016 Springer International Publishing AG (outside the USA)

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Pierce, C.L., Rees, J.C., Barr, J.R. (2016). Novel Approaches for Detection of Bacteriophage. In: Harper, D., Abedon, S., Burrowes, B., McConville, M. (eds) Bacteriophages. Springer, Cham. https://doi.org/10.1007/978-3-319-40598-8_20-1

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  • DOI: https://doi.org/10.1007/978-3-319-40598-8_20-1

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-40598-8

  • Online ISBN: 978-3-319-40598-8

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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