Skip to main content

Detection of Bacteriophages: Sequence-Based Systems


The invention of sequencing technologies has fundamentally changed molecular biology, including the way we look at bacteriophages. In addition to investigating bacteriophage plaques, electron micrographs, or the phenotypes of different mutants, we are now able to explore the entire genetic potential encoded in their genomes. As of July 2018, over 6,000 complete phage genome sequences were published in public databases, with over 28,000 partial sequences available.

In this chapter, we give an overview of the latest technologies that can be used to determine phage genome sequences, ranging from short-read platforms which generally give multiple gigabases of sequence data to long-read technologies which have the potential to sequence a bacteriophage genome in one single read. We then look at applications of sequencing technologies in detecting bacteriophages, from a single gene, over entire genomes, to the community level.

This is a preview of subscription content, access via your institution.

Fig. 1


Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Evelien M. Adriaenssens .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2019 Springer Nature Switzerland AG

About this entry

Verify currency and authenticity via CrossMark

Cite this entry

Owen, S.V., Perez-Sepulveda, B.M., Adriaenssens, E.M. (2019). Detection of Bacteriophages: Sequence-Based Systems. In: Harper, D., Abedon, S., Burrowes, B., McConville, M. (eds) Bacteriophages. Springer, Cham.

Download citation

  • DOI:

  • Received:

  • Accepted:

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-40598-8

  • Online ISBN: 978-3-319-40598-8

  • eBook Packages: Springer Reference Biomedicine & Life SciencesReference Module Biomedical and Life Sciences