The Identification of Skin Allergens by In Vivo Assay
In vivo assays for skin sensitization are valuable assets, but must not be regarded as 100% accurate.
Guinea pig methods have been superseded by the murine local lymph node assay (LLNA) which is now in the process of being replaced by nonanimal alternatives.
Regulatory classification as a skin sensitizing chemical means that the substance is regarded as significantly active.
A substance which is not classified as a skin sensitizer may still possess a limited potential to cause this effect.
Potency categories are new to regulatory sensitization, but the LLNA EC3 value provides a useful guide to the relative potency of a skin sensitizer.
KeywordsSkin sensitization Local lymph node assay Regulatory classification Potency categorization
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