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Aerobic Utilization of Hydrocarbons, Oils and Lipids pp 1–21Cite as

Structure-Function Relationships and Engineering of Haloalkane Dehalogenases

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Part of the book series: Handbook of Hydrocarbon and Lipid Microbiology ((HHLM))

Abstract

The structure-function relationships for haloalkane dehalogenases, one of the best characterized enzyme families involved in degradation of halogenated compounds, are described. A substantial amount of mechanistic and structural information is currently available on haloalkane dehalogenases, providing good theoretical framework for their modification by protein engineering. Examples of constructed mutants include variants with modified (i) activity and specificity, (ii) stability, and (ii) enantioselectivity. Many variants carried mutations in the tunnels connecting the buried active site with surrounding solvent, rather than in the active site itself. Mutagenesis of residues lining the protein tunnels represents attractive and a viable approach of protein engineering.

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Acknowledgments

Financial support is gratefully acknowledged from the Ministry of Education, Youth, and Sports of the Czech Republic (LO1214, LM2015051, LM2015047, LM2015055, LQ1605), The Czech Grant Agency (GA16-06096S, GA16-07965S, GA16-24223S), and the European Union H2020 EXCELERATE (676559). Access to the METACentrum supercomputing facilities is highly appreciated (LM2015042 and LM2015085).

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  1. Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00, Brno, Czech Republic

    Piia Kokkonen, Tana Koudelakova, Radka Chaloupkova, Lukas Daniel, Zbynek Prokop & Jiri Damborsky

  2. International Centre for Clinical Research, St. Anne’s University Hospital, Pekarska 53, 656 91, Brno, Czech Republic

    Piia Kokkonen, Tana Koudelakova, Radka Chaloupkova, Lukas Daniel, Zbynek Prokop & Jiri Damborsky

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  1. Piia Kokkonen
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  2. Tana Koudelakova
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Correspondence to Piia Kokkonen .

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  1. CSIC Campus de Cantoblanco, Centro Nacional de Biotecnología CSIC Campus de Cantoblanco, Madrid, Spain

    Fernando Rojo

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Kokkonen, P., Koudelakova, T., Chaloupkova, R., Daniel, L., Prokop, Z., Damborsky, J. (2017). Structure-Function Relationships and Engineering of Haloalkane Dehalogenases. In: Rojo, F. (eds) Aerobic Utilization of Hydrocarbons, Oils and Lipids. Handbook of Hydrocarbon and Lipid Microbiology . Springer, Cham. https://doi.org/10.1007/978-3-319-39782-5_15-1

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