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Metabolite Profiling of In Vitro Plant Systems

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Bioprocessing of Plant In Vitro Systems

Part of the book series: Reference Series in Phytochemistry ((RSP))

Abstract

Gas chromatography-mass spectrometry is one of the base analytical platforms used in plant metabolite profiling. The remarkable recent methodological and technological developments in GC-MS profiling expand the possibilities for its application in different fields of plant science including plant biotechnology. The methods of extraction, fractionation, derivatization, and metabolite identification, associated with GC-MS metabolite profiling, along with examples demonstrating the power and applicability of GC-MS in plant in vitro studies, have been presented in this chapter.

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Abbreviations

CE:

capillary electrophoresis

EI:

electron impact

GC:

gas chromatography

HPLC:

high performance liquid chromatography

HRMS:

high resolution mass spectrometry

LC:

liquid chromatography

LLE:

liquid-liquid extraction

MAE:

microwave-assisted extraction

MS:

mass spectrometry

NMR:

nuclear magnetic resonance

RI:

retention index

SPE:

solid phase extraction

SPME:

solid phase microextraction

TOF:

time of flight

UPLC:

ultrahigh performance liquid chromatography

UV:

ultraviolet

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Authors and Affiliations

  1. Institute of Biodiversity and Ecosystem Research at the Bulgarian Academy of Sciences, 2 Gagarin Str., Sofia, 1113, Bulgaria

    Strahil Berkov, Borjana Sidjimova & Milena Nikolova

  2. Agrobioinstitute, 8 Dragan Tzankov Str, Sofia, 1164, Bulgaria

    Liliya Georgieva

Authors
  1. Strahil Berkov
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  2. Liliya Georgieva
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  3. Borjana Sidjimova
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  4. Milena Nikolova
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Corresponding author

Correspondence to Strahil Berkov .

Editor information

Editors and Affiliations

  1. University of Food Technologies , Plovdiv, Bulgaria

    Atanas Pavlov

  2. Bioprocess Engineering, TU Dresden, Institute of Food Technology and Bioprocess Engineering, TU Dresden, Dresden, Germany

    Thomas Bley

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Berkov, S., Georgieva, L., Sidjimova, B., Nikolova, M. (2017). Metabolite Profiling of In Vitro Plant Systems. In: Pavlov, A., Bley, T. (eds) Bioprocessing of Plant In Vitro Systems. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-319-32004-5_12-1

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  • DOI: https://doi.org/10.1007/978-3-319-32004-5_12-1

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  • Print ISBN: 978-3-319-32004-5

  • Online ISBN: 978-3-319-32004-5

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