Bioreactor Technology for Hairy Roots Cultivation

Living reference work entry
Part of the Reference Series in Phytochemistry book series (RSP)


Bioreactor technology is an integral requisite to the development of scale-up production process of many plant-based high-value products. The proper selection and design of the bioreactor is required to determine the optimal industrial scale bioprocess and the subsequent capital investment. A primary cause of the lack of success in commercial production of secondary compounds using hairy root culture systems is their low yield. To increase the production of hairy root-based bioactive compounds, several strategies, like elicitation, metabolic engineering, and up-scaling, etc., have been adopted. Out of these strategies, the up-scaling in bioreactor deals with the principle of large-scale metabolite production in proportion of high biomass growth. The goal of an effective bioreactor is to control, contain, and positively influence a biological reaction in an incessant way in order to get desired productivity. This chapter provides a descriptive account on up-scaling of hairy root cultures for various purposes including secondary metabolite production. This chapter also discusses the hitherto reports on up-scaling of hairy root cultures of various plant species in terms of modifications in designing of bioreactors for incessant tissue growth concomitantly with metabolite production.


Agrobacterium rhizogenes Hairy roots Bioreactor designing Fermentor Scale-up Secondary metabolites Root clump Mass transfer 



Air lift reactors


Bubble column reactors


Hairy root cultures


Nutrient mist reactor


Stirred tank


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Copyright information

© Springer International Publishing AG 2016

Authors and Affiliations

  • Shakti Mehrotra
    • 1
  • Sonal Mishra
    • 2
  • Vikas Srivastava
    • 3
  1. 1.Plant Biotechnology DivisionCentral Institute of Medicinal and Aromatic PlantsLucknowIndia
  2. 2.National Institute of Plant Genome ResearchNew DelhiIndia
  3. 3.Department of Plant SciencesCentral University of JammuJammu (J & K)India

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