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Glucosinolates pp 383-405 | Cite as

Changing Trends in the Methodologies of Extraction and Analysis of Hydrolytic Products of Glucosinolates: A Review

  • Rohit AroraEmail author
  • Sakshi BhushanEmail author
  • Saroj AroraEmail author
Reference work entry
Part of the Reference Series in Phytochemistry book series (RSP)

Abstract

The increasing resistance among various pests and pathogens to the available synthetic medicines had lead to the compulsion for exploring new and improved alternatives. The phytochemicals have arisen as effective and safer alternate. Among the many plant secondary metabolites, glucosinolates surpass in biological activity and hence have been exhaustively extracted and explored for their activity against these dreaded diseases. This augmented demand for exploring the biological properties of all the available glucosinolates, especially their hydrolytic products, has lead to the development of new methods for extraction and analysis of these metabolites. The extraction methods are designed to match the volatility of the compound without degrading its quality. These methods have been improved to choose the best extraction conditions including the extracting solvents. The extracted glucosinolate hydrolytic products are further analyzed using an array of analytical methods ranging from as simple as paper chromatography to as complex as microchip analysis. These methods are designed and developed to match the needs of accuracy, reliability, and repeatability in addition to the cost effectiveness. This review thus is a highlight and a milestone for the scientists and budding researchers working on the crucial task of extracting, analyzing, and exploring the biological properties of glucosinolate hydrolytic products.

Keywords

Glucosinolate hydrolytic products Biosynthesis Hydrodistillation GC-MS HPLC Microchip analysis 

Abbreviations

AITC

Allyl isothiocyanate

DCM

Methylene chloride

ELISA

Enzyme linked immunosorbent assay

FID

Flame ionization detector

GC

Gas chromatography

GHPs

Glucosinolate hydrolytic products

GSLs

Glucosinolates

HILIC

Hydrophilic interaction liquid chromatography

HPLC

High performance liquid chromatography

HS

Head space

HSCCC

High speed counter-current chromatography

ITCs

Isothiocyanates

MS

Mass spectrometry

NIRS

Near infrared reflectance spectroscopy

NMR

Nuclear magnetic resonance

PAPS

3-phosphoadenosine-5′-phosphosufate

PC

Paper chromatography

S-GT: UDPG

Thiohydroximateglucosyl transferase

SIXCPC

Strong ion-exchange centrifugal partition chromatography

SIXCPE

Strong ion-exchange centrifugal partition extraction

TLC

Thin layer chromatography

UHPLC

Ultra high performance liquid chromatography

Notes

Acknowledgment

The study was supported by the funding provided by Department of Science and Technology (DST) and University Grants Commission (UGC), New Delhi and Guru Nanak Dev University, Amritsar.

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Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  1. 1.Department of Botanical and Environmental SciencesGuru Nanak Dev UniversityAmritsarIndia

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