Glucosinolates pp 431-461 | Cite as

Investigation of Glucosinolates by Mass Spectrometry

  • Giuliana BiancoEmail author
  • Raffaella PascaleEmail author
  • Filomena LelarioEmail author
  • Sabino A. BufoEmail author
  • Tommaso R. I. CataldiEmail author
Reference work entry
Part of the Reference Series in Phytochemistry book series (RSP)


There is an ever-increasing interest in the biological effects of glucosinolates (GSLs), due to their anticarcinogenic properties and potential to contribute to dietary phytonutrient consumption, thereby improving human health and well-being. Cruciferous vegetables are unique in that they are rich sources of GSLs. Several epidemiological studies have shown that a high intake of cruciferous vegetables, for example, cabbage, broccoli, or Brussels sprouts, has beneficial influence on human health. A new window of investigations has been opened by mass spectrometry (MS) and its combination with the various chromatographic techniques has proved to be highly successful to gain reliable data about the presence and abundance of GSLs in vegetables. The purpose of this chapter is focused on the methods that are currently available for their qualitative and quantitative analysis by MS and tandem MS (MS/MS). Emphasis is placed on the description and value of existing methods as well as on the many MS applications reported for GSL analysis.


Glucosinolates Isothiocyanate Mass spectrometry Tandem mass spectrometry High resolution 



Atmospheric pressure chemical ionization


Capillary electrophoresis


Collision-induced dissociation


Capillary zone electrophoresis


Direct analysis in real time


Electrospray ionization


Fast atom bombardment


Gas chromatography


Gas–liquid chromatography




High-performance capillary electrophoresis


High-performance liquid chromatography


High-resolution mass spectrometry


Infrared multiphoton dissociation




Ion trap mass spectrometry


Liquid chromatography


Liquid chromatography–electrospray ionization


Matrix-assisted laser desorption/ionization


Tandem mass spectrometry


Micellar electrokinetic capillary chromatography


Multiple reaction monitoring


Mass spectrometry


Mass spectrometry imaging


Nuclear magnetic resonance


Reversed-phase high-performance liquid chromatography


Selected ion monitoring


Selected reaction monitoring


Total ion current


Time of flight


Extracted ion chromatogram



This study was supported by the Università degli Studi di Bari Aldo Moro (Fondi di Ateneo 2012) and Università degli Studi della Basilicata.


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© Springer International Publishing Switzerland 2017

Authors and Affiliations

  1. 1.Dipartimento di ScienzeUniversità degli Studi della BasilicataPotenzaItaly
  2. 2.Scuola di IngegneriaDipartimento di Scienze, Università degli Studi della BasilicataPotenzaItaly
  3. 3.Dipartimento di Chimica and Centro Interdipartimentale SMARTUniversità degli Studi di Bari Aldo Moro, Campus UniversitarioBariItaly

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