Polyphenols in Herbal Extracts

Living reference work entry

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Part of the Reference Series in Phytochemistry book series (RSP)


Herbs are well known and often used in everyday life. This work is a review of commonly used herbs. The focus was on their short characteristics, therapeutic effects, and polyphenolic profile. The most popular methods of extracting polyphenolic compounds from herbs were compared. The main attention was paid to water and alcoholic extraction as they are used in domestic conditions. A separate section is devoted to fortification of beverages with herbs.


Polyphenols Herbs Herbal beverages Extraction Infusions Mint Chamomile Sage Thyme 


  1. 1.
    Barreira JCM, Morais AI, Ferreira ICFR, Oliviera MB (2013) Insights on the formulation of herbal beverages with medicinal claims according to their antioxidant properties. Molecules 18:2851–2863PubMedPubMedCentralGoogle Scholar
  2. 2.
    Carvalho-Costa D, Costa HS, Goncalves Albequerque T, Ramos F, Castilho MC, Sanches-Silva A (2015) Advances in phenolic compounds analysis of aromatic plants and their potential application. Trends Food Sci Technol 45:336–354Google Scholar
  3. 3.
    Chan EWC, Lim YY, Chong KI, Tan JBI, Wong SK (2012) Antioxidant properties of tropical and temperate herbal teas. J Food Compos Anal 23:185–189Google Scholar
  4. 4.
    Galleano M, Verstraeten SV, Oleiza PI, Fraga G (2010) Antioxidant actions of flavonoids: thermodynamic and kinetic analysis. Arch Biochem Biophys 401:23–30Google Scholar
  5. 5.
    Firenzuoli F, Gori L (2007) Herbal medicine today: clinical and research issues. Evid Based Complement Alternat Med 4(Suppl 1):37–40PubMedPubMedCentralGoogle Scholar
  6. 6.
    Sentkowska A, Biesaga M, Pyrzynska K (2015) Polyphenolic composition and antioxidative properties of lemon balm (Melissa officinalis L.) extract affected by different brewing processes. Int J Food Prop 18:2009–2014Google Scholar
  7. 7.
    Zhang QW, Lin LG, Ye WC (2018) Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med 13:20–46PubMedPubMedCentralGoogle Scholar
  8. 8.
    Barros L, Duenas M, Dias MI, Sousa MJ, Santos-Buelga C, Ferreira ICFR (2013) Phenolic profiles of cultivated, in vitro cultured and commercial samples of Melissa officinalis L. infusions. Food Chem 136:1–8PubMedGoogle Scholar
  9. 9.
    Enko J, Gliszczynska-Swiglo A (2015) Influence of the interactions between tea (Camellia sinensis) extracts and ascorbic acid on their antioxidant activity: analysis with interaction indexes and isobolograms. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 32:1234–1242PubMedGoogle Scholar
  10. 10.
    Sentkowska A, Pyrzynska K (2018) Zwitterionic hydrophilic interaction liquid chromatography coupled to mass spectrometry for analysis of beetroot juice and antioxidant interaction between its active compounds. LWT Food Sci Technol 93:641–648Google Scholar
  11. 11.
    Palafox-Carlos H, Gil-Chaves J, Sotelo-Mundo SS, Namieśnik J, Gorinstein S, Gonzales-Aguilar GA (2012) Antioxidant interaction between major polyphenolic compounds found in “Atafulo” mango pulp: chlorogenic, gallic, protocatechuic and vanillic acids. Molecules 17:12657–12664PubMedPubMedCentralGoogle Scholar
  12. 12.
    Sentkowska A, Pyrzynska K (2019) Evaluation of the antioxidant interactions between green tea polyphenols and nonsteroidal anti-inflammatory drugs. Open Chem J 6:47–51Google Scholar
  13. 13.
    Sentkowska A, Pyrzynska K (2018) Investigation of antioxidant interaction between green tea polyphenols and acetaminophen using isobolographic analysis. J Pharm Biomed Anal 159:393–397PubMedGoogle Scholar
  14. 14.
    Sentkowska A, Pyrzynska K (2020) Simultaneous determination of vitamin B6 and catechins in dietary supplements by ZIC-HILIC chromatography and their antioxidant interactions. Eur Food Res Technol.
  15. 15.
    Mouly S, Lioret-Linares C, Sellier PO, Sene D, Bergmann JF (2017) Is the clinical relevance of drug–food and drug–herb interactions limited to grapefruit juice and Saint-John’s wort? Pharmacol Res 118:82–92PubMedGoogle Scholar
  16. 16.
    Izzo AA, Hoon-Kim S, Radhakrishnan R, Williamson EM (2016) A critical approach to evaluating clinical efficacy, adverse events and drug interactions of herbal remedies. Phytother Res 30:691–700PubMedGoogle Scholar
  17. 17.
    Zhao J, Deng JW, Chen YW, Li SP (2013) Advanced phytochemical analysis of herbal tea in China. J Chromatogr A 1313:2–23PubMedGoogle Scholar
  18. 18.
    Carabajal MPA, Isla MI, Zampini IC (2017) Evaluation of antioxidant and antimutagenic activity of herbal teas from native plants used in traditional medicine in Argentina. S Afr J Bot 110:258–265Google Scholar
  19. 19.
    Jabria MA, Saklya M, Marzoukib L, Hichem Sebala H (2017) Chamomile (Matricaria recutita L.) decoction extract inhibits in vitro intestinal glucose absorption and attenuates high fat diet-induced lipotoxicity and oxidative stress. Biomed Pharmacother 87:153–159Google Scholar
  20. 20.
    Manayi A, Vazirian M, Saeidnia S (2015) Echinacea purpurea: pharmacology, phytochemistry and analysis methods. Pharmacogn Rev 9:63–72PubMedPubMedCentralGoogle Scholar
  21. 21.
    Castada HZ, Mirondo R, Sigurdson GT, Giusti MM, Barringer S (2017) Deodorization of garlic odor by spearmint, peppermint and chocolate mint leaves and rosmarinic acid. LWT Food Sci Technol 84:160–167Google Scholar
  22. 22.
    Jan NN, Zarafshan K, Singh S (2017) Stinging nettle (Urtica dioica L.): a reservoir of nutrition and bioactive components with great functional potential. J Food Meas Charact 11:423–433Google Scholar
  23. 23.
    Fritz H, Seely D, Flower G, Skidmore B, Fernandes R, Vadeboncoeur S, Kennedy D, Cooley K, Wong R, Sagar S, Sabri E, Fergusson D (2013) Soy, red clover, and isoflavones and breast cancer: a systematic review. PLoS One 8:e81968PubMedPubMedCentralGoogle Scholar
  24. 24.
    Patel S (2017) Rose hip as an underutilized functional food: evidence-based review. Trends Food Sci Technol 63:29–38Google Scholar
  25. 25.
    Hamidpour M, Hamidpour R, Hamidpour S, Shahlari M (2014) Chemistry, pharmacology and medicinal property of sage (Salvia) to prevent and cure illnesses such as obesity, diabetes, depression, dementia, lupus, autism, heart disease and cancer. J Tradit Complement Med 4:82–88PubMedPubMedCentralGoogle Scholar
  26. 26.
    Salehi B, Abu-Darwishb MS, Tarawnehc AH, Cabrald C, Gadetskayag AV, Salgueiroh L, Hosseinabadii T, Rajabij S, Chandak W, Sharifi-Radl M, Mulaudzim RB, Ayatollahin SA, Farzad Kobarfardn F, Arserim-Ucarr DK, Sharifi-Rads J, Atap A, Baghalpourn N, Contrerast MM (2019) Thymus spp. plants – food applications and phytopharmacy properties. Trends Food Sci Technol 85:287–306Google Scholar
  27. 27.
    Finimundy TC, Pereira C, Dias MI, Caleja C, Calhelha RC, Sokovic M, Stojković D, Carvalho AM, Rosa E, Barros L, Ferreira ICFR (2020) Infusions of herbal blends as promising sources of phenolic compounds and bioactive properties. Molecules 25:2151PubMedCentralGoogle Scholar
  28. 28.
    Guimarães R, Barros L, Dueňas M, Calhelka RC, Carvalho AM, Santos-Buelga C, Queiroz MJ, Ferreira ICF (2013) Infusion and decoction of wild German chamomile: bioactivity and characterization of organic acids and phenolic compounds. Food Chem 136:947–954PubMedGoogle Scholar
  29. 29.
    Vinholes J, Vizzotto M (2017) Synergisms in alpha-glucosidase inhibition and antioxidant activity of Camellia sinensis L. Kuntze and Eugenia uniflora L. ethanolic extracts. Pharmacogn Res 9:101–107Google Scholar
  30. 30.
    Che CT, Wang ZJ, Chow MS, Lam CWK (2013) Herb–herb combination for therapeutic enhancement and advancement: theory, practice and future perspectives. Molecules 18:5125–5141PubMedPubMedCentralGoogle Scholar
  31. 31.
    Riehle P, Vollmer M, Rohn S (2012) Phenolic compounds in Cistus incanus herbal infusions – antioxidant capacity and thermal stability during brewing process. Food Res Int 53:891–899Google Scholar
  32. 32.
    Zhang QW, Lin LG, Ye WC (2018) Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med 13:20–46PubMedPubMedCentralGoogle Scholar
  33. 33.
    Azmin SNHM, Manan ZA, Alwi SB, Chua LS, Mustaffa AA, Yunus NA (2016) Herbal processing and extraction technologies. Sep Purif Rev 45:305–320Google Scholar
  34. 34.
    Yahyaa NA, Attana N, Wahab RA (2018) An overview of cosmeceutically relevant plan extracts and strategies for extraction of plant-based bioactive compounds. Food Bioprod Process 112:69–85Google Scholar
  35. 35.
    Belwal T, Ezzat SM, Rastrelli L, Bhatt ID, Daglia M, Baldi A, Devkota HP, Orhan IE, Patra JK, Das G, Anandharamakrishnan C, Gomez LG, Nabavi SF, Nabavi SM, Atanasov AG (2018) A critical analysis of extraction techniques used for botanicals: trends, priorities, industrial uses and optimization strategies. TrAC Trends Anal Chem 100:82–102Google Scholar
  36. 36.
    Vinatoru M, Mason TJ, Calinescu I (2017) Ultrasonically assisted extraction (UAE) and microwave assisted extraction (MAE) of functional compounds from plant materials. TrAC Trends Anal Chem 97:159–178Google Scholar
  37. 37.
    Belwal T, Chemat F, Venskutonis PR, Cravotto G, Jaiswal DK, Bhatt ID, Devkota HP, Luo Z (2020) Recent advances in scaling-up of non-conventional extraction techniques: learning from successes to failures. TrAC Trends Anal Chem 127:15895Google Scholar
  38. 38.
    Plaza M, Marina ML (2019) Pressurized hot water extraction of bioactives. TrAC Trends Anal Chem 116:236–247Google Scholar
  39. 39.
    Dróżdż P, Sentkowska A, Pyrzynska K (2016) Calluna vulgaris (L.) hull as a source of chlorogenic acid. Acad J Med Plants 12:75–80Google Scholar
  40. 40.
    McAlpine MD, Ward WE (2016) Influence on sleeping time on polyphenols content and antioxidant capacity of black, green, rooibos and herbal teas. Beverages 2:17–29Google Scholar
  41. 41.
    Albuquerque BR, Pieto MA, Barreiro MF, Rodrigues A, Curran TP, Barros L, Fereira ICFR (2017) Catechin-based extract optimization obtained from Arbutus unedo L. fruits using maceration/microwave/ultrasound extraction techniques. Ind Crop Prod 95:404–415Google Scholar
  42. 42.
    Jovanović AA, Dordevic VB, Zdunic GM, Pljevljakusic DS, Savikin KP, Godevac DM, Bugarski BM (2017) Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat and ultrasound assisted techniques. Sep Purif Technol 179:369–390Google Scholar
  43. 43.
    Sentkowska A, Biesaga M, Pyrzynska K (2016) Effects of brewing process on phenolic compounds and antioxidant activity of herbs. Food Sci Biotechnol 25:965–970PubMedPubMedCentralGoogle Scholar
  44. 44.
    Kratachanova M, Denev P, Ciz M, Lojek A, Atanas Mihailov A (2010) Evaluation of antioxidant activity of medicinal plants containing polyphenol compounds. Comparison of two extraction systems. Acta Biochim Pol 57:229–234Google Scholar
  45. 45.
    Dias MI, Barros L, Sousa MJ, Ferreira ICF (2012) Systematic comparison of nutraceuticals and antioxidant potential of cultivated, in vitro cultured and commercial Melissa officinalis samples. Food Chem Toxicol 50:1866–1873PubMedGoogle Scholar
  46. 46.
    Fotakis C, Tsigrimani D, Tsiaka T, Lantzouraki DZ, Strati IF, Makris C, Tagkouli D, Proestos C, Sinanoglou VJ, Zoumpoulakis P (2016) Metabolic and antioxidant profiles of herbal infusions and decoctions. Food Chem 211:963–971PubMedGoogle Scholar
  47. 47.
    Ajila CM, Brar SK, Verma M, Tyagi RD, Godbout S, Valero JR (2011) Extraction and analysis of polyphenols: recent trends. Crit Rev Anal Chem 31:227–249Google Scholar
  48. 48.
    Acosta-Estrada BA, Gutierrez-Uribe JA, Serna-Saldivar SO (2014) Bound phenolics in foods, a review. Food Chem 152:46–55PubMedGoogle Scholar
  49. 49.
    Marete EN, Jacquier JC, O’Riordan D (2009) Effects of extraction temperature on the phenolic and parthenolide contents, and colour of aqueous feverfew (Tanacetum parthenium) extracts. Food Chem 117:226–231Google Scholar
  50. 50.
    Dawidowicz AL, Typek R (2010) Thermal stability of 5-o-caffeoylquinic acid in aqueous solutions at different heating conditions. J Agric Food Chem 58:12578–12584PubMedGoogle Scholar
  51. 51.
    Arya V, Thakur NM, Kashyap C (2012) Preliminary phytochemical analysis of the extracts of Psidium leaves. J Pharmacogn Phytochem 1:1–5Google Scholar
  52. 52.
    Al-Mansoub MA, Asmawi MZ, Murugaiyah V (2014) Effects of extraction solvents and plant parts used on the antihyperlipidemic and antioxidant effects of Garcinia atroviridis: a comparative study. J Sci Food Agric 94:1552–1558PubMedGoogle Scholar
  53. 53.
    Pavithra K, Vadivukkarasi S (2015) Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Sci Human Wellness 4:42–46Google Scholar
  54. 54.
    Dróżdż P, Sentkowska A, Pyrzynska K (2017) Flavonoid content and antioxidant properties in different extracts of Calluna vulgaris (L.) flowers. J Agric Sci Technol A 7:39–44Google Scholar
  55. 55.
    Dent M, Dragovic-Uzelac V, Penic M, Brncic M, Bosilijkov T, Levaj B (2013) The effect of extraction solvents, temperature and time on the composition and mass fraction of polyphenols in Dalmatin wild sage (Salvia officinalis L.) extracts. Food Technol Biotechnol 51:84–91Google Scholar
  56. 56.
    Waszkowiak K, Gliszczyńska-Świgło A (2016) Binary ethanol–water solvents affects phenolic profile and antioxidant capacity of flaxseed extracts. Eur Food Res Technol 242:777–786Google Scholar
  57. 57.
    Marques V, Farah A (2009) Chlorogenic acids and related compounds in medicinal plants and infusions. Food Chem 113:1370–1376Google Scholar
  58. 58.
    Kala HK, Mehta R, Sen KK, Tandey R, Mandal V (2016) Critical analysis of research trends and issues in microwave assisted extraction of polyphenolics: have we really done enough. Trends Anal Chem 85:140–152Google Scholar
  59. 59.
    Kumoro C, Hartari I (2015) Microwave assisted extraction of dioscorin from Gadung (Dioscorea Hispidia Dennst) Tuber Flour. Procedia Chem 14:47–55Google Scholar
  60. 60.
    Hartari I, Kurniasari L, Anas Y (2015) Mathematical model of the hydrotropic microwave assisted extraction of antimalarial agent from Andrographis paniculata. Procedia Chem 14:186–192Google Scholar
  61. 61.
    Kufmann B, Christen P (2002) Recent extraction techniques for natural products: microwave-assisted extraction and pressurized solvent extraction. Phytochem Anal 13:105–113Google Scholar
  62. 62.
    Dent M, Gragovic-Uzelac V, Garofulic IE, Bosiljkov T, Jezek D, Brncic M (2015) Comparison of conventional and ultrasound assisted techniques on mass fraction of phenolic compounds from sage (Salivia officinalis L.). Chem Biochem Eng 29:475–484Google Scholar
  63. 63.
    Xiao J, Chen G, Li N (2018) Ionic liquid solutions as a green tool for the extraction and isolation of natural products. Molecules 23:1765PubMedCentralGoogle Scholar
  64. 64.
    Lou Z, Wang H, Zhu S, Chen S, Zhang M, Wang Z (2012) Ionic liquids based simultaneous ultrasonic and microwave assisted extraction of phenolic compounds from burdock leaves. Anal Chim Acta 715:28–33Google Scholar
  65. 65.
    Yang Z, Tan Z, Li F, Li X (2016) An effective method for the extraction and purification of chlorogenic acid from ramie (Boehmeria nivea L.) leaves using acidic ionic liquids. Ind Corps Prod 89:78–86Google Scholar
  66. 66.
    Jordan A, Gathergood N (2015) Biodegradation of ionic liquids – a critical review. Chem Soc Rev 44:8200–8237PubMedGoogle Scholar
  67. 67.
    Li XY, Jing CQ, Lei WL, Li J, Wang JJ (2012) Apoptosis caused by imidazolium-based ionic liquids in PC12 cells. Ecotoxicol Environ Saf 83:102–107PubMedGoogle Scholar
  68. 68.
    Kudłak B, Owczarek K, Namieśnik J (2015) Selected issues related to the toxicity of ionic liquids and deep eutectic solvents – a review. Environ Sci Pollut Res 22:11975–11992Google Scholar
  69. 69.
    Skarpalezos D, Detsi A (2019) Deep eutectic solvents as extraction media for valuable flavonoids from natural sources. Appl Sci 9:4169Google Scholar
  70. 70.
    Wei Z, Qi X, Li T, Luo M, Wang W, Zu Y, Fu Y (2015) Application of natural deep eutectic solvents for extraction and determination of phenolics in Cajanus cajan leaves by ultra performance liquid chromatography. Sep Purif Technol 149:237–244Google Scholar
  71. 71.
    Wei ZF, Wang XQ, Peng X, Wang W, Zhao CJ, Gang Zu Y, Fu YJ (2015) Fast and green extraction and separation of main bioactive flavonoids from Radix Scutellariae. Ind Crop Prod 63:175–181Google Scholar
  72. 72.
    Ansari MM, Kumar DS (2012) Fortification of food and beverages with phytonutrients. Food Pub Health 2:241–253Google Scholar
  73. 73.
    Jaeger SR, Axten LG, Wohlers MW, Sun-Waterhouse D (2009) Polyphenol-rich beverages: insights from sensory and consumer science. J Sci Food Agric 89:2356–2362Google Scholar
  74. 74.
    Malongane F, McGaw LJ, Mudau FN (2017) The synergistic potential of various teas, herbs and therapeutic drugs in health improvement: a review. J Sci Food Agric 97:4679–4689PubMedGoogle Scholar
  75. 75.
    Sentkowska A, Pyrzynska K (2019) Investigation of antioxidant activity of selenium compounds and their mixtures with tea polyphenols. Mol Biol Rep 46:3019–3024PubMedGoogle Scholar
  76. 76.
    Freeman BL, Eggett DL, Parker TL (2010) Synergistic and antagonistic interactions of phenolic compounds found in navel oranges. J Food Sci 75:C570–C576PubMedGoogle Scholar
  77. 77.
    Tamer CE, Yekeler FZ, Çopur ÖU, İncedayi B, Suna S (2017) A study of fortification of lemonade with herbal extracts. Food Sci Technol 37:45–51Google Scholar
  78. 78.
    Sharma SK, Yadav VK, Rao VK, Dixit AK (2014) Enhancement of health-promoting properties while substituting exogenous citric acid by seabuckthorn (Hippophae salicifolia) pulp in preparation of sweet orange (Citrus sinensis) ready-to-serve beverage. J Food Process Preserv 38:1427–143.8Google Scholar
  79. 79.
    Sanchez-Bel P, Romojaro A, Egea I, Pretel MT (2015) Wild edible plants as potential antioxidant or nutritional supplements for beverages minimally processed. LWT Food Sci Technol 62:830–837Google Scholar
  80. 80.
    Ivanišová E, Frančáková H, Ritschlová P, Dráb Š, Solgajová M, Tokár M (2015) Biological activity of apple juice enriched by herbal extracts. J Microbiol Biotechnol Food Sci 4:69–73Google Scholar
  81. 81.
    Shon MS, Lee Y, Song JH, Park T, Lee JK, Kim M, Park E, Kim GN (2014) Anti-aging potential of extracts prepared from fruits and medicinal herbs cultivated in the Gyeongnam area of Korea. Prev Nutr Food Sci 19:178–186PubMedPubMedCentralGoogle Scholar

Authors and Affiliations

  1. 1.Heavy Ion LaboratoryUniversity of WarsawWarsawPoland
  2. 2.Department of ChemistryUniversity of WarsawWarsawPoland

Section editors and affiliations

  • K. G. Ramawat
    • 1
  1. 1.Department of BotanyUniversity College of Science, M. L. Sukhadia UniversityUdaipurIndia

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