Antioxidant Activity and Capacity Measurement

Living reference work entry
Part of the Reference Series in Phytochemistry book series (RSP)


Measurement of antioxidant activity/capacity of foods and biological fluids is important for the determination of nutritional value of foods and for the diagnosis of diseases associated with oxidative stress. Antioxidant activity/capacity can be monitored using assays with different mechanisms, including (i) electron transfer-based assays, (ii) hydrogen atom transfer-based assays, (iii) mixed-mode assays, (iv) lipid peroxidation assays, (v) ROS/RNS scavenging assays, (vi) oxidative stress biomarkers and cellular-based assays, and (vii) chromatographic, chemometric, and electrochemical assays. In this chapter, the mechanisms of the most widely used analytical approaches for the measurement of antioxidant activity/capacity are discussed together with their advantages and drawbacks. Overall, measurement of antioxidant activity/capacity, in particular for mixtures, multifunctional or complex multiphase systems, cannot be evaluated satisfactorily with a simple antioxidant assay due to the several variables affecting the results. Therefore, it is recommended to apply several assays with different mechanisms in order to reveal the full capability of an antioxidant sample.


Antioxidant activity measurement Electron transfer-based assays Hydrogen atom transfer-based assays Mixed-mode assays Lipid peroxidation assays ROS/RNS scavenging assays Oxidative stress biomarkers Cellular-based assays Chromatographic assays Chemometric assays Electrochemical assays 


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Authors and Affiliations

  1. 1.Department of Food Engineering, Faculty of Chemical and Metallurgical EngineeringIstanbul Technical UniversityMaslak, IstanbulTurkey
  2. 2.Science and Technology Application and Research Center (BITUAM)Bursa Uludag UniversityGorukle, BursaTurkey
  3. 3.Department of Chemistry, Faculty of EngineeringIstanbul University-CerrahpasaAvcilar, IstanbulTurkey
  4. 4.Turkish Academy of Sciences (TUBA)Cankaya, AnkaraTurkey

Section editors and affiliations

  • Jaya Arora
    • 1
  1. 1.Laboratory of Biomolecular Technology, Department of BotanyMohanlal Sukhadia UniversityUdaipurIndia

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